Development of immobilized peroxidase on amino-functionalized magnetic MgFe2O4 nanoparticles for antioxidant activity and decolorization

IF 2.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biophysical chemistry Pub Date : 2025-03-01 Epub Date: 2024-12-04 DOI:10.1016/j.bpc.2024.107366

Keziban Atacan , Nuray Güy , Alican Bahadir Semerci , Mahmut Özacar

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Abstract

This investigation aims to immobilize peroxidase onto 3-aminopropyltriethoxysilane (APTES)-functionalized MgFe₂O₄ magnetic nanoparticles to increase enzyme stability, efficiency, and recyclability. The synthesized samples were analyzed using X-ray diffraction, Fourier transform infrared spectroscopy, Thermogravimetric analysis, Vibrating sample magnetometer, and Scanning electron microscopy. The free and immobilized peroxidase were examined against different pH and temperatures as well as storage time and reuse. The immobilized peroxidase maintained 52 % of its initial activity after 10 consecutive measurements thanks to easy magnetic separation. In addition, antioxidant activity was increased by immobilizing the peroxidase to the MgFe₂O₄ magnetic nanoparticles. Congo red dye removal for peroxidase immobilized MgFe₂O₄-APTES was found to be 98.6 % for 240 min. Also, it showed approximately two times more dye decolorization efficiency compared to MgFe₂O₄ and APTES modified MgFe₂O₄. Finally, the immobilized peroxidase was studied by a decolorization study of congo red. So, we believe that the immobilized peroxidase on magnetic nanoparticles reported in this study may be utilized in diverse biotechnology, industrial, and environmental practices.

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氨基功能化磁性MgFe2O4纳米颗粒固定化过氧化物酶的研制及其抗氧化脱色性能。

本研究旨在将过氧化物酶固定在3-氨基丙基三乙氧基硅烷（APTES）功能化的MgFe2O4磁性纳米颗粒上，以提高酶的稳定性、效率和可回收性。利用x射线衍射、傅里叶变换红外光谱、热重分析、振动样品磁强计和扫描电镜对合成的样品进行了分析。考察了游离过氧化物酶和固定化过氧化物酶对不同pH、温度、贮存时间和重复使用的影响。由于易于磁分离，在连续10次测量后，固定化过氧化物酶保持了52%的初始活性。此外，通过将过氧化物酶固定在磁性纳米颗粒上，可以提高氧化镁的抗氧化活性。过氧化物酶固定化MgFe2O4-APTES对刚果红染料的去除率为98.6%，脱色率约为MgFe2O4和APTES改性MgFe2O4的两倍。最后，对固定化过氧化物酶进行了刚果红脱色研究。因此，我们相信本研究报道的磁性纳米颗粒固定化过氧化物酶可用于各种生物技术，工业和环境实践。

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来源期刊

Biophysical chemistry 生物-生化与分子生物学

CiteScore

6.10

自引率

10.50%

发文量

121

审稿时长

20 days

期刊介绍： Biophysical Chemistry publishes original work and reviews in the areas of chemistry and physics directly impacting biological phenomena. Quantitative analysis of the properties of biological macromolecules, biologically active molecules, macromolecular assemblies and cell components in terms of kinetics, thermodynamics, spatio-temporal organization, NMR and X-ray structural biology, as well as single-molecule detection represent a major focus of the journal. Theoretical and computational treatments of biomacromolecular systems, macromolecular interactions, regulatory control and systems biology are also of interest to the journal.