Effects of nanopolystyrene and/or phoxim exposure on digestive function of Eriocheir sinensis

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-12-07 DOI:10.1016/j.cbpc.2024.110102
Mengting Huang , Yuan Ma , Qianru Fan , Shunli Che , Jun Zhang , Shuquan Ding , Shuren Zhu , Xilei Li
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Abstract

Nanopolystyrene (NP) and phoxim (PHO) are pervasive environmental contaminants that pose a significant threat to the health of aquatic organisms, prompting widespread concern among researchers and the public alike. The hepatopancreas play important roles in the Chinese mitten crab (Eriocheir sinensis), such as digestion, absorption and detoxification. This study assessed the hepatopancreatic toxicity caused by the exposure of Eriocheir sinensis to environmentally relevant concentrations of NP and/or PHO. After a 21-day exposure period, NP (1.0 × 1010 particles/L) and PHO (24 μg/L) exposure resulted in reduced number of blister-like, resorptive, and fibrillar cells and an elevation in lipid droplets within the hepatopancreas compared to the control group. Furthermore, trypsin and lipase activity decreased, amylase activity increased, and a significantly decrease in the expression of digestion-related genes, including CHT, CarL, and CarB, suggested impairment in both digestive and metabolic functions. The marked upregulation of key genes, including PPARγ, GYK, PEPCK, and SCD, as well as key metabolites such as 4-methylzymosterol-carboxylate, zymosterone, lathosterol, 7-dehydro-desmosterol, vitamin D2, 24-methylene-cycloartanol, 5-dehydroepisterol, and sitosterol in the lipid metabolic pathway, showed that the peroxisome proliferator-activated receptor (PPAR) and steroid biosynthesis signaling pathways were highly affected by exposure to NP and/or PHO. These findings indicated that exposure to NP and/or PHO might adversely affect the hepatopancreatic physiological homeostasis in E. sinensis by causing tissue damage and interfering with digestive and metabolic functions. Our results provide ecotoxicological insights into the effects of nanopolystyrene and/or phoxim exposure on the digestive function of Eriocheir sinensis.

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纳米聚苯乙烯和辛硫磷暴露对中华绒螯蟹消化功能的影响。
纳米聚苯乙烯(NP)和硫辛(PHO)是普遍存在的环境污染物,对水生生物的健康构成重大威胁,引起了研究人员和公众的广泛关注。肝胰腺在中华绒螯蟹(Eriocheir sinensis)的消化、吸收和排毒等方面起着重要的作用。本研究评估了中华绒螯蟹暴露于环境相关浓度的NP和/或PHO所引起的肝胰毒性。在21天的暴露期后,与对照组相比,NP(1.0 × 1010 颗粒/L)和PHO(24 μg/L)暴露导致肝胰脏内水泡样细胞、吸收细胞和纤维细胞数量减少,脂滴升高。此外,胰蛋白酶和脂肪酶活性降低,淀粉酶活性升高,消化相关基因(包括CHT、CarL和CarB)的表达显著降低,表明消化和代谢功能受损。在脂质代谢途径中,PPARγ、GYK、PEPCK和SCD等关键基因以及4-甲基酶甾醇-羧酸盐、酶酮、乙醇醇、7-脱氢去氨甾醇、维生素D2、24-亚甲基环artanol、5-脱氢episterol和谷甾醇等关键代谢产物的显著上调表明,过氧化物酶体增殖激活受体(PPAR)和类固醇生物合成信号通路受到NP和/或PHO暴露的高度影响。这些结果表明,暴露于NP和/或PHO可能通过引起组织损伤和干扰消化代谢功能而对中华鄂仔鸡肝胰腺生理稳态产生不利影响。我们的研究结果为纳米聚苯乙烯和/或辛硫磷暴露对中华绒螯蟹消化功能的影响提供了生态毒理学见解。
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来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
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