Quantitative analysis of vasicine and vasicinone in Adhatoda vasica (Nees.) using RP-HPLC: distribution and solvent extraction efficiency

IF 1.6 3区化学 Q3 CHEMISTRY, APPLIED Natural Product Research Pub Date : 2025-11-02 DOI:10.1080/14786419.2024.2440521

C. Rohini , T.A. Kavya , A. Niranjana Kumar , J. Kotesh Kumar , K.V.N. Satya Srinivas , B. Venkatesh , P. Ravi Kumar

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Abstract

Adhatoda vasica Nees (Acanthaceae), an ayurvedic shrub, is used widely in respiratory disorders. The pharmacologically most studied phytoconstituent in A. vasica is vasicine, a bitter quinazoline alkaloid present in leaves. A robust method was developed for the simultaneous quantification and analysis of two major alkaloids i.e. vasicine and vasicinone by RP-HPLC. This paper describes the distribution of these markers in different parts of the plant along with the best extraction method for their recovery. The highest abundance of vasicine was observed in the leaves (0.7332%), than in the stems and roots, whereas vasicinone was present only in the leaves (0.0436%). Methanol was identified as the most effective solvent for the extraction of vasicine in successive extraction (the herb was extracted sequentially with increasing solvent polarity) whereas 30% aq. Methanol is recommended for extraction of vasicinone in individual solvent extraction (the fresh herb was extracted separately with different solvents) method.

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利用 RP-HPLC 法定量分析 Adhatoda vasica (Nees.) 中的 vasicine 和 vasicinone：分布和溶剂萃取效率。

阿育吠陀灌木（刺科），广泛用于呼吸系统疾病。药理学上研究最多的植物成分是缬草碱，一种存在于叶片中的苦喹唑啉生物碱。建立了反相高效液相色谱法（RP-HPLC）同时定量分析水杨碱和水杨碱酮两种主要生物碱的方法。本文介绍了这些标记物在植物不同部位的分布，以及提取它们的最佳方法。水杨花苷在叶片中的丰度最高（0.7332%），其次是茎和根，而水杨花苷酮仅在叶片中存在（0.0436%）。在连续提取法中，甲醇是提取水仙花酮最有效的溶剂（随着溶剂极性的增加，水仙花酮被依次提取），而在单溶剂提取法中，建议使用30% aq的甲醇提取水仙花酮（用不同的溶剂分别提取新鲜草药）。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.