Environmental release behavior, cell toxicity and intracellular distribution of novel biodegradable plastic materials

Abstract

In response to the increasingly severe issue of plastic waste, biodegradable plastics have garnered extensive attention as a potential alternative to traditional plastics. Among these materials, biodegradable plastics hold a dominant position. The objective of this study was to assess the environmental risks of five commercially available biodegradable plastics: polyglycolic acid (PGA), polylactic acid (PLA), poly(butylene succinate) (PBS), poly(butylene carbonate) (PBC), and poly(butylene adipate-co-terephthalate) (PBAT). The evaluation included their physical properties, microplastic release behavior, and cytotoxicity. In addition, the effect of age process on the environmental behavior of biodegradable plastic materials was further investigated. The results revealed that PGA and PBS exhibited lower risks in terms of microplastic release, whereas PLA demonstrated higher environmental mobility. Further cytotoxicity experiments indicated that PLA and PBS exerted significant toxic effects on human cell lines, including human normal liver cells (LO2), human monocytic leukemia cells (THP-1), human umbilical vein endothelial cells (HUVECs), and human colon carcinoma cells (Caco-2). Additionally, this study utilized Nile Red labeling to observe the co-culture system of PGA with THP-1 cells, uncovering that THP-1 cells gradually engulfed and internalized PGA microplastics over time. This finding provides new insights into the potential mechanism by which microplastics promote cell proliferation. Moreover, we also found that the aging process partially reduced the cytotoxicity of PGA, but had little effect on environmental mobility. Considering the comprehensive research findings, PGA is considered an ideal material for large-scale applications due to its low cytotoxicity and environmental risks. In contrast, the environmental safety of other types of plastics requires more comprehensive risk assessment to determine their suitability. This study provides significant scientific evidence for the environmental impact assessment of biodegradable plastics and plays a crucial role in promoting the development of sustainable plastic alternatives.