Pop-Up Paper-Based Biosensor for a Dual-Mode Lung Cancer ctDNA Assay Based on Novel CoB Nanosheets with Dual-Enzyme Activities and a Portable Smartphone/Barometer for Readout

IF 8.2 1区 化学 Q1 CHEMISTRY, ANALYTICAL ACS Sensors Pub Date : 2024-12-18 DOI:10.1021/acssensors.4c01470
Siyi Yang, Jiajia Zhu, Liyu Yang, Huanbao Fa, Yongzhong Wang, Danqun Huo, Changjun Hou, Daidi Zhong, Mei Yang
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Abstract

Timely monitoring of circulating tumor DNA (ctDNA) in serum is meaningful for personalized diagnosis and treatment for lung cancer. Cheap and efficient point-of-care testing (POCT) has emerged as a promising method, especially in a low-resource setting. Herein, (i) a 3D pop-up paper-based POCT device was designed and manufactured via a cheap method; it was used for saving time and efficiently building a biosensor; (ii) a novel cobalt boride nanosheet (CoB NS) nanozyme with abundant groups was used for POCT dual-mode signal transduction and then a portable smartphone/pressure meter to readout; (iii) a user-friendly smartphone app was fabricated for achieving more convenient POCT. Detailly, the dual-mode signal generated was based on the CoB NS with peroxidase activity to catalyze a chromogenic agent to develop color and with catalase activity to catalyze decomposition of H2O2 to O2. Density functional theory (DFT) and experimental results showed a good catalysis performance of the CoB NS via studying its five possible catalytic pathways, in which the metal Co is the catalytic active site center that acts as the electron donor and promotes electron transfer between the CoB NS and the adsorbed substrates. Benefiting from that, the proposed method showed good analytical ability in detecting ctDNA. Besides, its accuracy was valued by comparing it with the standard qPCR method to detect real samples from tumor cells and tumor-bearing mice, which showed a consistent result and potential practical applicability of the proposed method for POCT ctDNA. In general, this work not only provided a dual-mode POCT platform that could also be applied for other analytes but also first revealed the nanozyme properties of the CoB NS and inspired its new application from electrocatalysis, energy, etc. to biomedicine.

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基于具有双酶活性的新型CoB纳米片和用于读数的便携式智能手机/气压计的Pop-Up纸质生物传感器用于肺癌ctDNA双模式检测
及时监测血清循环肿瘤DNA (ctDNA)对肺癌的个性化诊断和治疗具有重要意义。廉价和高效的即时检测(POCT)已成为一种有前途的方法,特别是在资源匮乏的环境中。本文(i)采用廉价的方法设计并制造了3D弹出式纸质POCT装置;它被用来节省时间和有效地建立一个生物传感器;(ii)利用具有丰富基团的新型硼化钴纳米片(CoB NS)纳米酶进行POCT双模信号转导,然后使用便携式智能手机/压力计进行读数;(iii)制作了用户友好的智能手机应用程序,以实现更方便的POCT。具体而言,所产生的双模信号是基于CoB NS具有过氧化物酶活性,催化显色剂显色,并具有过氧化氢酶活性,催化H2O2分解为O2。密度功能理论(DFT)和实验结果通过对CoB NS的5种可能催化途径的研究表明,CoB NS具有良好的催化性能,其中金属Co作为催化活性位点中心,作为电子供体,促进CoB NS与吸附底物之间的电子转移。因此,该方法对ctDNA的检测具有良好的分析能力。此外,通过将其与标准qPCR方法检测肿瘤细胞和荷瘤小鼠的真实样本进行比较,对其准确性进行了评价,结果一致,表明该方法对POCT ctDNA具有潜在的实用性。总的来说,这项工作不仅提供了一个可以应用于其他分析物的双模POCT平台,而且首次揭示了CoB NS的纳米酶性质,并激发了其从电催化、能源等到生物医学的新应用。
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来源期刊
ACS Sensors
ACS Sensors Chemical Engineering-Bioengineering
CiteScore
14.50
自引率
3.40%
发文量
372
期刊介绍: ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.
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