In-situ synthesis of FeS nanoparticles enhances Sulfamethoxazole degradation via accelerated electron transfer in anaerobic bacterial communities

Abstract

The impact of nanominerals on microbial electron transfer and energy metabolism strategies during pollutant degradation remains uncertain. This study used in situ synthesized FeS nanoparticles (FeS NPs) to increase the degradation efficiency of SMX by anaerobic bacterial communities from 25.80 % to 47.60 %. The proportion of intracellular degradation by bacteria in the community significantly increased by 23.25 times, which mainly facilitated by NADH-dependent reductases and iron-sulfur proteins. Microbial network analysis and electrochemical analysis indicated that the in-situ synthesis of FeS NPs altered the interactions among different microbial species, enabling Petrimonas to transfer electrons directly to Lysinibacillus more effectively. This adjustment led to an increase in the activity of the electron transport system by 1.2 times, an increase in the electron supply capacity by 2.8 times, and a decrease in the electrochemical impedance (EIS) to 3.21 Ω. Moreover, the coupling of electron transfer pathways and protease transport channels significantly increased Na⁺/K⁺-ATPase by 14.72 times. Inhibitor experiments and molecular dynamics (MD) results showed that FeS NPs interact with Nqo1 in the cell membrane via electrostatic force at -28.573 kcal/mol, forming a unique electron conduit with ubiquinone (CoQ). This study provides new insights into the role of in situ nanominerals in electron transfer between different microorganisms, aim to enhance the antibiotic wastewater treatment efficiency in actual anaerobic processes.