Is Pseudofrankia, the non-nitrogen-fixing and/or non-nodulating actinorhizal nodule dweller, mutualistic or parasitic? Insights from genome-predictive analysis.

Abstract

This study re-evaluates Pseudofrankia strains, traditionally regarded as parasitic dwellers of actinorhizal root nodules due to their inability to fix nitrogen (Fix -) and/or nodulate (Nod -), as potential plant growth-promoting bacteria (PGPB). We compared plant growth-promoting traits (PGPTs) between Pseudofrankia strains, including one newly sequenced strain BMG5.37 in this study and typical (Fix + /Nod +) Frankia, Protofrankia, and Parafrankia, as well as non-frankia actinorhizal species Nocardia and Micromonospora, and the phytopathogenic Streptomyces. Although lacking nitrogen-fixing genes typically found in mutualistic Frankiaceae strains, Pseudofrankia may compensate through predicted pathways for denitrification and nitrate utilization. Functional profiling suggests potential for phosphorus solubilization, gibberellin production, and vitamin metabolism, as well as bioremediation of pollutants. Pseudofrankia strains are predicted to show moderate resistance to heavy metals, with a stronger tolerance to arsenic and tellurium compared to Frankia. Furthermore, they are anticipated to exhibit significant biotic and abiotic stress resistance, including oxidative and osmotic stress. Predictive data also indicate that Pseudofrankia strains may have root colonization abilities and may play a role in plant signaling and phytohormone production, particularly in auxin and gibberellin pathways. Secretion systems, especially CE-Type VI, are predicted to be highly developed in Pseudofrankia, suggesting potential for effective plant interactions. These findings position Pseudofrankia strains as promising candidates for plant growth promotion, although experimental validation and the integration of transcriptomic or proteomic data are needed to confirm these predictions.