CA1 ensembles expressing immediate-early genes are driven by context switch, shrink with sustained presence, and show no effect of change of task demands

IF 2.6 3区心理学 Q2 BEHAVIORAL SCIENCES Behavioural Brain Research Pub Date : 2024-12-20 DOI:10.1016/j.bbr.2024.115407

Branislav Krajcovic , Daniela Cernotova , Helena Buchtova , Ales Stuchlik , Stepan Kubik , Jan Svoboda

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Abstract

The hippocampus (HPC) is essential for navigation and memory, tracking environmental continuity and change, including navigation relative to moving targets. CA1 ensembles expressing immediate-early gene (IEG) Arc and Homer1a RNA are contextually specific. While IEG expression correlates with HPC-dependent task demands, the effects of behavioral demands on IEG-expressing ensembles remain unclear. In three experiments, we investigated the effects of context switch, sustained presence, and task demands on dorso-proximal CA1 IEG+ ensembles in rats.

Experiment 1 showed that the size of IEG+ (Arc, Homer1a RNA) ensembles dropped to baseline during uninterrupted 30-min exploration, reflecting familiarization, unless a context switch was present. Context-specificity of the ensembles depended on both environment identity and timing of the context switch. Experiment 2 found no effect of HPC-dependent mobile robot avoidance or HPC-independent avoidance of a stationary robot on IEG+ ensembles beyond mere exploration. Experiment 3 replicated these findings for c-Fos protein.

The data suggest that IEG+ ensembles are driven by a context switch and shrink over time during sustained presence in the same environment. We found no evidence of task demands or their change affecting the size, stability over time, or task-specificity of IEG+ ensembles.

These results shed light on the temporal dynamics of CA1 IEG+ ensembles, and their control by contextual and behavioral factors.

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表达即时早期基因的CA1基因群受环境切换驱动，随着持续存在而缩小，且不受任务需求变化的影响。

海马体（HPC）对于导航和记忆，跟踪环境的连续性和变化，包括相对于移动目标的导航至关重要。表达直接早期基因（IEG） Arc和Homer1a RNA的CA1集合具有上下文特异性。虽然IEG表达与hpc依赖的任务需求相关，但行为需求对IEG表达集合的影响尚不清楚。在三个实验中，我们研究了上下文切换、持续存在和任务需求对大鼠背-近端CA1 IEG+组合的影响。实验1显示，在不间断的30分钟探索期间，IEG+ (Arc, Homer1a RNA)集合的大小下降到基线，反映了熟悉程度，除非存在上下文切换。集合的上下文特异性取决于环境身份和上下文切换的时间。实验2发现，除了单纯的探索外，依赖于hpc的移动机器人回避或不依赖于hpc的固定机器人回避对IEG+整体没有影响。实验3在c-Fos蛋白中重复了这些发现。数据表明，IEG+组合是由上下文切换驱动的，并且在同一环境中持续存在时随着时间的推移而缩小。我们没有发现任务需求或它们的变化会影响IEG+集合的大小、稳定性或任务特异性的证据。这些结果揭示了CA1 IEG+群落的时间动态，以及它们受环境和行为因素的控制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Behavioural Brain Research 医学-行为科学

CiteScore

5.60

自引率

0.00%

发文量

383

审稿时长

61 days

期刊介绍： Behavioural Brain Research is an international, interdisciplinary journal dedicated to the publication of articles in the field of behavioural neuroscience, broadly defined. Contributions from the entire range of disciplines that comprise the neurosciences, behavioural sciences or cognitive sciences are appropriate, as long as the goal is to delineate the neural mechanisms underlying behaviour. Thus, studies may range from neurophysiological, neuroanatomical, neurochemical or neuropharmacological analysis of brain-behaviour relations, including the use of molecular genetic or behavioural genetic approaches, to studies that involve the use of brain imaging techniques, to neuroethological studies. Reports of original research, of major methodological advances, or of novel conceptual approaches are all encouraged. The journal will also consider critical reviews on selected topics.