Simulated Gastrointestinal Fluids Impact the Stability of Polymer-Functionalized Selenium Nanoparticles: Physicochemical Aspects.

IF 6.5 2区医学 Q1 NANOSCIENCE & NANOTECHNOLOGY International Journal of Nanomedicine Pub Date : 2024-12-17 eCollection Date: 2024-01-01 DOI:10.2147/IJN.S483253

Atiđa Selmani, Nives Matijaković Mlinarić, Salvatore Fabio Falsone, Ivan Vidaković, Gerd Leitinger, Ida Delač, Borna Radatović, Ivan Nemet, Sanda Rončević, Andreas Bernkop-Schnürch, Tomislav Vuletić, Karin Kornmueller, Eva Roblegg, Ruth Prassl

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Abstract

Background: Selenium (Se) is a vital micronutrient for maintaining homeostasis in the human body. Selenium nanoparticles (SeNPs) have demonstrated improved bioavailability compared to both inorganic and organic forms of Se. Therefore, supplementing with elemental Se in its nano-form is highly promising for biomedical applications related to Se deficiency.

Purpose: The primary objective of this study was to evaluate the impact of the main gastrointestinal proteins on the physicochemical properties and stability of polymer-coated SeNPs.

Methods: SeNPs functionalized with thiolated chitosan or hyaluronic acid were characterized based on their composition, morphology, size, and zeta potential. The stability of these particles was evaluated in simulated gastric and intestinal fluids. Additionally, the interaction propensity between major gastric proteins, such as pepsin and pancreatin, and functionalized SeNPs was investigated with FTIR, fluorescence quenching titrations, and in situ adsorption measurements.

Results: The composition of the media, including pH and ionic strength, the chemistry of polymers, and the presence of the proteins, influence the size and zeta potential of the SeNPs. The increase in NP size due to the formation of large agglomerates, along with the decrease in zeta potential magnitude, confirmed the formation of a protein corona. Both pepsin and pancreatin showed a strong affinity to the particle surface. Based on the values of the apparent equilibrium dissociation constant this affinity was more pronounced for positively charged thiolated chitosan coated SeNPs compared to those coated with negatively charged hyaluronic acid. The polymer coated SeNPs displayed antioxidative potential, which could be very beneficial for health conditions associated with Se-deficiency.

Conclusion: This study highlights the importance of exploring the characteristics of polymer-functionalized SeNPs under gastrointestinal conditions. Such investigations are important for developing nutritional supplements that can gradually release Se from SeNPs, thereby improving selenium absorption, bioavailability, and safety.

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模拟胃肠道液体影响聚合物功能化硒纳米颗粒的稳定性：物理化学方面。

背景：硒（Se）是维持人体内平衡的重要微量元素。与无机和有机形式的硒相比，硒纳米颗粒（SeNPs）已经证明了更好的生物利用度。因此，补充硒元素的纳米形式在与硒缺乏相关的生物医学应用中具有很大的前景。目的：本研究的主要目的是评估主要胃肠道蛋白对聚合物包被SeNPs的物理化学性质和稳定性的影响。方法：利用巯基壳聚糖或透明质酸对SeNPs进行结构、形态、大小和zeta电位表征。在模拟胃液和肠液中评估了这些颗粒的稳定性。此外，通过FTIR、荧光猝灭滴定和原位吸附测量，研究了胃蛋白酶和胰蛋白酶等主要胃蛋白与功能化SeNPs之间的相互作用倾向。结果：培养基的组成，包括pH值和离子强度，聚合物的化学性质，以及蛋白质的存在，都会影响senp的大小和zeta电位。由于形成大团簇而增加的NP大小，以及zeta电位大小的降低，证实了蛋白质电晕的形成。胃蛋白酶和胰蛋白酶均与颗粒表面有较强的亲和力。根据表观平衡解离常数的值，与带负电荷的透明质酸包覆的SeNPs相比，带正电荷的硫代壳聚糖包覆的SeNPs具有更明显的亲和性。聚合物包被的SeNPs显示出抗氧化潜力，这可能对与缺硒相关的健康状况非常有益。结论：本研究强调了探索胃肠道条件下聚合物功能化SeNPs特征的重要性。这些研究对于开发能够从SeNPs中逐渐释放硒的营养补充剂，从而提高硒的吸收、生物利用度和安全性具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Nanomedicine NANOSCIENCE & NANOTECHNOLOGY-PHARMACOLOGY & PHARMACY

CiteScore

14.40

自引率

3.80%

发文量

511

审稿时长

1.4 months

期刊介绍： The International Journal of Nanomedicine is a globally recognized journal that focuses on the applications of nanotechnology in the biomedical field. It is a peer-reviewed and open-access publication that covers diverse aspects of this rapidly evolving research area. With its strong emphasis on the clinical potential of nanoparticles in disease diagnostics, prevention, and treatment, the journal aims to showcase cutting-edge research and development in the field. Starting from now, the International Journal of Nanomedicine will not accept meta-analyses for publication.