A label-free electrochemical biosensor for sensitive analysis of the PARP-1 activity.

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-12-26 DOI:10.1016/j.bioelechem.2024.108891

Haitang Yang, Siming Pu, Penghua Shu, Jiapan Wang, YuYu Chen, Xinshuo Yang, Yuce Hou, Wei Wei

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Abstract

Early diagnosis of tumors is becoming increasingly important in modern healthcare. As studies have demonstrated, Poly(ADP)ribose polymerase-1 (PARP-1) is overexpressed in more aggressive tumors. Consequently, sensitive detection of PARP-1 activity holds significant practical importance in clinical diagnostics and biomedical research. Herein, an electrochemical biosensor for the sensitive monitoring of the PARP-1 activity have been proposed. The presence of target PARP-1 firstly triggers enzyme-initiated auto-PARylation and formed negatively charged polymer consisting of a few to 200 ADP-ribose units. Due to electrostatic adsorption, negatively charged PAR will bind with a large number of positively charged methylene blue (MB) electroactive molecules. By detecting the electrochemical signal of MB on the indium tin oxide (ITO) electrode, PARP-1 activity detection was achieved with a linear detection range of 0-1.0 U and a detection limit as low as 0.003 U. The proposed biosensor shows great prospects of clinical application.

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用于PARP-1活性敏感分析的无标记电化学生物传感器。

肿瘤的早期诊断在现代医疗保健中变得越来越重要。研究表明，聚（ADP）核糖聚合酶-1 （PARP-1）在侵袭性更强的肿瘤中过表达。因此，PARP-1活性的灵敏检测在临床诊断和生物医学研究中具有重要的实际意义。本文提出了一种用于灵敏监测PARP-1活性的电化学生物传感器。目标PARP-1的存在首先触发酶启动的自parp化，形成由几个到200个adp核糖单位组成的带负电荷的聚合物。由于静电吸附作用，带负电的PAR会与大量带正电的亚甲基蓝（MB）电活性分子结合。通过在氧化铟锡（ITO）电极上检测MB的电化学信号，实现了PARP-1活性的检测，线性检测范围为0-1.0 U，检出限低至0.003 U，该生物传感器具有广阔的临床应用前景。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.