Insertion of the β-ketoacyl-CoA synthase MdKCS2 promoter segment causes wax biosynthesis difference in apple peel

IF 8.1 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2025-01-08 DOI:10.1111/nph.20378

Lizhi Zhang, Shaobo Yin, Mingqian Wang, Zhi Liu, Tianxing Lv, Yanan Wang, Aide Wang, Dongmei Tan, Yinglin Ji

{"title":"Insertion of the β-ketoacyl-CoA synthase MdKCS2 promoter segment causes wax biosynthesis difference in apple peel","authors":"Lizhi Zhang, Shaobo Yin, Mingqian Wang, Zhi Liu, Tianxing Lv, Yanan Wang, Aide Wang, Dongmei Tan, Yinglin Ji","doi":"10.1111/nph.20378","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <ul>\n \n <li>Cuticular wax is essential for fruit to maintain moisture. Although the wax content of peel surface in apple (Malus spp.) varies, the detailed molecular mechanism remains unclear.</li>\n \n <li>Here, we identified the β-ketoacyl-CoA synthase 2 (MdKCS2) differentially expressed between apple peel with low and high wax content by integrating bulked segregant analysis-sequencing and RNA-seq. We found that a 63-bp insertion in the MdKCS2 promoter was the primary reason for apple peel with low wax content. The 63-bp insertion reduced MdKCS2 promoter activity and enhanced the DNA binding with the suppressor MdDOF4.6, decreasing wax biosynthesis by reducing C24 very-long-chain fatty acid (VLCFA).</li>\n \n <li>ECERIFERUM 2 (MdCER2) was co-expressed with MdKCS2 and suppressed by MdMYB56, MdbHLH137 and MdDOF4.6, further decreasing C29 alkane content in apple peel with low C24 VLCFA content.</li>\n \n <li>Overall, MdKCS2 and MdCER2 are coordinately involved in the wax production of apple peel surface.</li>\n </ul>\n </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 5","pages":"2069-2084"},"PeriodicalIF":8.1000,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.20378","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Cuticular wax is essential for fruit to maintain moisture. Although the wax content of peel surface in apple (Malus spp.) varies, the detailed molecular mechanism remains unclear.
Here, we identified the β-ketoacyl-CoA synthase 2 (MdKCS2) differentially expressed between apple peel with low and high wax content by integrating bulked segregant analysis-sequencing and RNA-seq. We found that a 63-bp insertion in the MdKCS2 promoter was the primary reason for apple peel with low wax content. The 63-bp insertion reduced MdKCS2 promoter activity and enhanced the DNA binding with the suppressor MdDOF4.6, decreasing wax biosynthesis by reducing C24 very-long-chain fatty acid (VLCFA).
ECERIFERUM 2 (MdCER2) was co-expressed with MdKCS2 and suppressed by MdMYB56, MdbHLH137 and MdDOF4.6, further decreasing C29 alkane content in apple peel with low C24 VLCFA content.
Overall, MdKCS2 and MdCER2 are coordinately involved in the wax production of apple peel surface.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

β-酮酰基辅酶a合成酶MdKCS2启动子片段的插入导致苹果皮蜡生物合成差异

果皮蜡是水果保持水分的必要物质。虽然苹果（Malus spp.）果皮表面蜡含量不同，但其具体的分子机制尚不清楚。在此，我们通过整合散装分离分析-测序和RNA-seq，鉴定了低蜡含量和高蜡含量苹果皮中β-酮酰基辅酶a合成酶2 （MdKCS2）的差异表达。我们发现MdKCS2启动子中63-bp的插入是苹果皮蜡含量低的主要原因。63 bp的插入降低了MdKCS2启动子活性，增强了与抑制子MdDOF4.6的DNA结合，通过减少C24甚长链脂肪酸（VLCFA）减少了蜡的生物合成。ECERIFERUM 2 （MdCER2）与MdKCS2共表达，并被MdMYB56、MdbHLH137和MdDOF4.6抑制，进一步降低了C24 VLCFA含量较低的苹果皮中C29烷烃含量。综上所述，MdKCS2和MdCER2协同参与苹果果皮表面蜡的生成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.