Insertion of the β-ketoacyl-CoA synthase MdKCS2 promoter segment causes wax biosynthesis difference in apple peel

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2025-01-08 DOI:10.1111/nph.20378

Lizhi Zhang, Shaobo Yin, Mingqian Wang, Zhi Liu, Tianxing Lv, Yanan Wang, Aide Wang, Dongmei Tan, Yinglin Ji

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引用次数: 0

Abstract

Cuticular wax is essential for fruit to maintain moisture. Although the wax content of peel surface in apple (Malus spp.) varies, the detailed molecular mechanism remains unclear.
Here, we identified the β-ketoacyl-CoA synthase 2 (MdKCS2) differentially expressed between apple peel with low and high wax content by integrating bulked segregant analysis-sequencing and RNA-seq. We found that a 63-bp insertion in the MdKCS2 promoter was the primary reason for apple peel with low wax content. The 63-bp insertion reduced MdKCS2 promoter activity and enhanced the DNA binding with the suppressor MdDOF4.6, decreasing wax biosynthesis by reducing C24 very-long-chain fatty acid (VLCFA).
ECERIFERUM 2 (MdCER2) was co-expressed with MdKCS2 and suppressed by MdMYB56, MdbHLH137 and MdDOF4.6, further decreasing C29 alkane content in apple peel with low C24 VLCFA content.
Overall, MdKCS2 and MdCER2 are coordinately involved in the wax production of apple peel surface.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.