Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfaces

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL Journal of Materials Science: Materials in Medicine Pub Date : 2025-01-07 DOI:10.1007/s10856-024-06849-0

Hisanobu Kamio, Kazuto Okabe, Masaki Honda, Kensuke Kuroda, Shuhei Tsuchiya

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Abstract

Osseointegration is essential for successful implant treatment. However, the underlying molecular mechanisms remain unclear. In this study, we focused on decorin (DCN), which was hypothesized to be present in the proteoglycan (PG) layer at the interface between bone and the titanium oxide (TiOx) surface. We utilized DCN RNA interference in human bone marrow mesenchymal stem cells (hBMSCs) to investigate its effects on PG layer formation, proliferation, initial adhesion, cell extension, osteogenic capacity, fibrotic markers, and immunotolerance to TiOx in vitro. After 14 days of cultivation, we observed no PG layer was detected, and the osteogenic capacity was suppressed in DCN-depleted hBMSCs. Furthermore, the conditioned medium upregulated the expression of M1 macrophage markers in human macrophages. These results suggest that endogenous DCN plays a crucial role in PG layer formation and that the PG layer alters inflammation around Ti materials.

Graphical Abstract

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人骨髓间充质干细胞中decorin的敲低抑制蛋白聚糖层的形成并在氧化钛表面建立促炎环境

骨整合是成功种植体治疗的关键。然而，潜在的分子机制尚不清楚。在这项研究中，我们重点研究了decorin (DCN)，它被假设存在于骨和氧化钛（TiOx）表面交界面的蛋白聚糖（PG）层中。我们利用DCN RNA干扰人骨髓间充质干细胞（hBMSCs），研究其对PG层形成、增殖、初始粘附、细胞扩展、成骨能力、纤维化标志物和体外对TiOx免疫耐受的影响。培养14天后，我们观察到未检测到PG层，并且在dcn缺失的hBMSCs中，成骨能力受到抑制。此外，条件培养基上调了人巨噬细胞中M1巨噬细胞标志物的表达。这些结果表明，内源性DCN在PG层的形成中起着至关重要的作用，PG层改变了Ti材料周围的炎症。图形抽象

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.