In vitro efficacy of levonadifloxacin against methicillin-resistant Staphylococcus aureus (MRSA) including hVISA isolates collected across India.

IF 1.4 4区 医学 Q4 IMMUNOLOGY Indian Journal of Medical Microbiology Pub Date : 2025-01-08 DOI:10.1016/j.ijmmb.2025.100788
Sneha Vinayan, Sujatha Sistla, Meerabai Manoharan, Kamini Walia, Madhan Sugumar
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Abstract

Background: Vancomycin has been the preferred treatment for MRSA infections. However, newer drugs are necessary due to the increasing prevalence of MRSA isolates that are less susceptible to vancomycin. Levonadifloxacin and its prodrug alalevonadifloxacin, novel quinolones with broad spectrum anti-MRSA activity. They are approved in India for the treatment of skin and soft-tissue infections, bacteraemia, and diabetic foot infections.

Materials and methods: A total of 456 MRSA (Methicillin-resistant Staphylococcus aureus) isolates, including 333 isolates from JIPMER and 123 isolates from other tertiary care centers collected between January to December 2022, underwent susceptibility testing to levonadifloxacin by disc diffusion method. The study isolates included 29 h-VISA. Fifty one blood isolates were also subjected to E-test and their results were compared to the gold standard, broth micro dilution (BMD).

Result: All 456 isolates were found to be susceptible to levonadifloxacin by disc diffusion. Levonadifloxacin demonstrated potent activity against MRSA strains isolated from blood (n = 51) with an MIC50 and MIC90 of 0.38 and 0.5 μg/mL by E-test, respectively. Similarly, it demonstrated an MIC50 and MIC90 of 0.5 & 1 μg/ml by BMD.

Conclusion: The present study demonstrated 100 % susceptibility to levonadifloxacin among the tested isolates of MRSA, highlighting its promising clinical potential for treating infections caused by this organism. Furthermore, the study recorded a concordance of 100 % between disc diffusion and Etest method with BMD, indicating the reliability of these methods.

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左氧氟沙星对耐甲氧西林金黄色葡萄球菌(MRSA)的体外疗效,包括在印度收集的hVISA分离株。
背景:万古霉素是治疗MRSA感染的首选药物。然而,由于对万古霉素不太敏感的MRSA分离株日益流行,需要更新的药物。具有广谱抗mrsa活性的新型喹诺酮类药物左旋那沙星及其前药阿来那沙星。它们在印度被批准用于治疗皮肤和软组织感染、菌血症和糖尿病足感染。材料与方法:采用圆盘扩散法对2022年1 - 12月收集的456株MRSA(耐甲氧西林金黄色葡萄球菌)进行左旋那氟沙星药敏试验,其中JIPMER医院采集的333株和其他三级医疗中心采集的123株。研究分离了29个h-VISA。对51株血分离株进行e试验,并与金标准肉汤微量稀释法(BMD)进行比较。结果:456株分离菌均对左旋那地沙星敏感。左旋那沙星对51株血源MRSA有较强的抑制作用,E-test检测其MIC50和MIC90分别为0.38和0.5 μg/mL。BMD测定的MIC50和MIC90分别为0.5和1 μg/ml。结论:本研究显示,被检测的MRSA分离株对左旋那沙星的敏感性为100%,这表明其在治疗由MRSA引起的感染方面具有良好的临床潜力。此外,该研究记录了椎间盘扩散和测试方法与BMD之间100%的一致性,表明这些方法的可靠性。
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来源期刊
CiteScore
2.20
自引率
0.00%
发文量
154
审稿时长
73 days
期刊介绍: Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study. Review articles, Special Articles or Guest Editorials are accepted on invitation.
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