A combination of genome mining with OSMAC strategy facilitates the discovery of bioactive metabolites produced from termite-associated Streptomyces tanashiensis BYF-112

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2025-01-10 DOI:10.1002/ps.8640

Jun Wu, YiHeng Wang, YaXuan Wang, XiaoHong Li, YueYue Li, Miao Zhang, JianHao Xiong, CaiPing Yin, ShuXiang Zhang, XinHua Liu, YingLao Zhang

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Abstract

BACKGROUND

Previously, eight new alkaloids were obtained from the fermentation extract of termite-associated Streptomyces tanashiensis BYF-112. However, genome analysis indicated the presence of many undiscovered secondary metabolites in S. tanashiensis BYF-112.

RESULTS

Herein, 12 new alkaloids, tianwuine A–E (1–5), cephalandole C (6), venezuelines I–L (7–10), N-(4-methylphenyl-2-hydroxy) formamide (11) and N-(5-formyl-2-hydroxyphenyl) formamide (12), as well as three known metabolites (13–15) were discovered from BYF-112 based on a combination of genome mining and the one strain many compounds (OSMAC) strategy. Plausible biosynthetic pathways of 1–13 were proposed using bioinformatic analysis of the full genome of BYF-112. Partial metabolites were evaluated in vitro for their antibacterial, phytotoxic, and anti-inflammatory activities. Pyrroloformamide A (14) showed strong antibacterial activities against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Pseudomonas syringae pv. actinidae, Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola at a concentration of 50 μg per 6 mm disk. Simultaneously, pyrroloformamide A (14) also had a strong inhibitory effect on the radicle growth of Echinochloa crusgalli with an inhibition rate of 98.01% at a concentration of 100 μg/mL, equivalent to the positive 2,4-dichlorophenoxyacetic acid. Subsequently, the possible herbicidal mechanism of 14 was explored using molecular docking simulation. In addition, venezueline G (13) displayed a strong inhibitory effect of NO production, with an half-maximal inhibitory concentration (IC₅₀) value of 2.3 μm, which was comparable with that of BAY 11-7082 with an IC₅₀ value of 2.1 μm.

CONCLUSION

These findings revealed a perspective for the development of novel bioactive drugs in the food, agricultural, and biomedical fields utilizing the metabolites of BYF-112. © 2025 Society of Chemical Industry.

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基因组挖掘与OSMAC策略的结合促进了白蚁相关链霉菌tanashiensisBYF‐112产生的生物活性代谢物的发现

此前，从白蚁相关的tanashiensis BYF‐112发酵提取物中获得了8种新的生物碱。然而，基因组分析表明，在S. tanashiensis BYF‐112中存在许多未被发现的次级代谢物。结果基于基因组挖掘和一株多化合物（OSMAC）策略，从BYF - 112中共发现了12种新的生物碱，分别为天牛碱a - e（1-5）、头孢菌素C(6)、螺螺I-L（7-10）、N‐（4‐甲基苯基‐2‐羟基）甲酰胺（11）和N‐（5‐甲酰基‐2‐羟基）甲酰胺（12），以及3种已知的代谢物（13-15）。利用对BYF‐112全基因组的生物信息学分析，提出了1-13可能的生物合成途径。体外评估部分代谢物的抗菌、植物毒性和抗炎活性。吡咯甲酰胺A（14）对金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、丁香假单胞菌均有较强的抑菌活性。米黄单胞菌；米黄单胞菌和米黄单胞菌。Oryzicola浓度为50 μg / 6mm。同时，吡咯甲酰胺A（14）在浓度为100 μg/mL时，对刺青藻根生长也有较强的抑制作用，抑制率为98.01%，与阳性的2,4‐二氯苯氧乙酸相当。随后，通过分子对接模拟，探讨了14可能的除草机理。此外，委内林G（13）对NO的产生具有较强的抑制作用，其半抑制浓度（IC50）值为2.3 μm，与BAY 11‐7082的IC50值为2.1 μm相当。结论这些发现为利用BYF - 112的代谢物开发食品、农业和生物医学领域的新型生物活性药物提供了前景。©2025化学工业协会。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.