Exacerbation of virulence of multi-drug resistant Escherichia coli O104:H4 by subinhibitory concentrations of ampicillin.

IF 2.5 4区生物学 Q3 MICROBIOLOGY Research in microbiology Pub Date : 2025-01-08 DOI:10.1016/j.resmic.2025.104266

Yaraymi Ortiz, Vianey Lechuga, Carolina Ortiz, Eduardo Palomino, Eduardo Franco, Norma Heredia, Santos García

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Abstract

Little is known about how subinhibitory concentrations of antibiotics to which bacteria are resistant affect bacterial virulence. In this study, the effect of subinhibitory concentrations of ampicillin on the virulence of E. coli O104:H4 was analyzed. Bacteria were pre-exposed to 0.1, 0.3, or 0.5 mg/mL of ampicillin in LB media and incubated for 4 h at 37 °C. Transformation capacity (using plasmids and PCR-amplified DNA sequences), swarming motility, biofilm production, curli formation, and virulence gene expression were determined. Ampicillin increased the transformation of E. coli O104:H4, with the highest number of transformants (>10⁴ CFU/ng DNA; p ≤ 0.05) detected after exposure to DNA sequences of spectinomycin. In addition, bacteria pre-treated with 0.5 mg/mL of ampicillin exhibited higher swarming motility (7.6 cm, vs 6.0 cm for control; p ≤ 0.05) and biofilm production (up to 1.9-fold; p ≤ 0.05) when subsequently exposed to 0.1 and 0.3 mg/mL of antibiotic compared with the control. Also, significant overexpression of the virulence-related genes flhC (≤16.1-fold), fliA (≤22.1-fold), csgA (≤3.6-fold), csgD (≤9.1-fold), stx2a (≤32.2-fold), and the antibiotic resistance gene blaTEM-1 (≤5.5-fold) was observed. In conclusion, ampicillin-resistant E. coli O104:H4 increased the expression of its virulence factors when exposed to most subinhibitory concentrations of ampicillin analyzed in this study.

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氨苄西林亚抑制浓度加重多重耐药大肠杆菌O104:H4的毒力。

对于细菌耐药的抗生素的亚抑制浓度如何影响细菌的毒力，人们知之甚少。本研究分析了氨苄西林亚抑制浓度对大肠杆菌O104:H4毒力的影响。细菌在LB培养基中预先暴露于0.1、0.3或0.5 mg/mL氨苄西林中，在37℃下孵育4小时。检测转化能力（利用质粒和pcr扩增的DNA序列）、蜂群运动、生物膜生成、卷曲形成和毒力基因表达。氨苄西林增加了大肠杆菌O104:H4的转化，转化体数量最高(>104 CFU/ng DNA；p≤0.05)。此外，用0.5 mg/mL氨苄西林预处理的细菌表现出更高的群体能动性(7.6 cm，对照组为6.0 cm；P≤0.05)和生物膜产量(可达1.9倍；p≤0.05)，与对照组相比，随后暴露于0.1和0.3 mg/mL抗生素。毒力相关基因flhC（≤16.1倍）、fliA（≤22.1倍）、csgA（≤3.6倍）、csgD（≤9.1倍）、stx2a（≤32.2倍）和抗生素耐药基因blatem1（≤5.5倍）均显著过表达。综上所述，耐氨苄西林大肠杆菌O104:H4在暴露于本研究分析的大多数亚抑制浓度氨苄西林时，其毒力因子的表达增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.