Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential.

IF 1.5 Q3 SURGERY Plastic and Reconstructive Surgery Global Open Pub Date : 2025-01-10 eCollection Date: 2025-01-01 DOI:10.1097/GOX.0000000000006420

Karina Karina, Johannes A Biben, Krista Ekaputri, Grady Krisandi, Imam Rosadi, Siti Sobariah, Irsyah Afini, Tias Widyastuti, Alfida Zakiyah, Difky Ernanda

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Abstract

Background: The use of fat grafting has expanded to include cell and tissue regeneration, necessitating investigations to ensure the viability of stromal and adipose-derived mesenchymal stem cells (ASCs) within the transferred fat parcels. This study explored the impact of harvesting technique and centrifugation on the viability of stromal cells and ASCs in lipoaspirate.

Methods: Fat was harvested from patients undergoing fat grafting using 2 types of liposuction cannula: (A) a 3-mm blunt tip cannula with 3 smooth holes and (B) a 2.4-mm, sharp point port, multihole blunt tip cannula. Fat from cannula B underwent different processing methods: no centrifugation, 300g, 600g, and 900g centrifugation. Stromal cells were isolated, quantified, and evaluated for viability. ASCs were cultured from these samples to confirm survival.

Results: Lipoaspirates from 21 patients were analyzed. The mean stromal cell counts were 0.937 × 10⁹ ± 0.346 × 10⁹/mL for cannula A and 0.734 × 10⁹ ± 0.266 × 10⁹/mL for cannula B (P = 0.684), with viabilities of 98.79% and 98.22% (P = 0.631), respectively. ASCs isolated and after 2-passage culture were also higher for cannula A. Stromal cell quantification and viability were lowest in the noncentrifuged group (P < 0.05) and highest in the 600g centrifugation group.

Conclusions: Fat harvesting using cannulas A and B showed no significant difference in stromal cell yield or viability. Handheld syringe liposuction preserved stromal vascular fraction cell and ASC viability. Centrifugation at different speeds did not significantly affect stromal cell viability.

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重新探讨脂肪移植收获和加工技术以优化其再生潜力。

背景：脂肪移植的应用已经扩展到包括细胞和组织再生，需要研究以确保移植脂肪包内基质和脂肪来源的间充质干细胞（ASCs）的活力。本研究探讨了采收技术和离心对抽脂液中基质细胞和ASCs活力的影响。方法：采用2种吸脂套管(A) 3孔3mm钝头套管和(B) 2.4 mm尖口多孔钝头套管，对脂肪移植术患者进行脂肪收集。B套管脂肪的处理方法不同：不离心、300g、600g、900g离心。分离基质细胞，定量并评估其活力。从这些样本中培养ASCs以确认存活。结果：对21例患者的抽脂液进行了分析。A套管间质细胞平均计数为0.937 × 109±0.346 × 109/mL， B套管间质细胞平均计数为0.734 × 109±0.266 × 109/mL (P = 0.684)，存活率分别为98.79%和98.22% （P = 0.631）。间质细胞的数量和活力以未离心组最低（P < 0.05），以600g离心组最高（P < 0.05）。结论：A套管和B套管在基质细胞产量和活力方面无显著差异。手持注射器吸脂保存间质血管部分细胞和ASC活力。不同离心速度对基质细胞活力无显著影响。

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来源期刊

Plastic and Reconstructive Surgery Global Open SURGERY-

CiteScore

2.20

自引率

13.30%

发文量

1584

审稿时长

10 weeks

期刊介绍： Plastic and Reconstructive Surgery—Global Open is an open access, peer reviewed, international journal focusing on global plastic and reconstructive surgery.Plastic and Reconstructive Surgery—Global Open publishes on all areas of plastic and reconstructive surgery, including basic science/experimental studies pertinent to the field and also clinical articles on such topics as: breast reconstruction, head and neck surgery, pediatric and craniofacial surgery, hand and microsurgery, wound healing, and cosmetic and aesthetic surgery. Clinical studies, experimental articles, ideas and innovations, and techniques and case reports are all welcome article types. Manuscript submission is open to all surgeons, researchers, and other health care providers world-wide who wish to communicate their research results on topics related to plastic and reconstructive surgery. Furthermore, Plastic and Reconstructive Surgery—Global Open, a complimentary journal to Plastic and Reconstructive Surgery, provides an open access venue for the publication of those research studies sponsored by private and public funding agencies that require open access publication of study results. Its mission is to disseminate high quality, peer reviewed research in plastic and reconstructive surgery to the widest possible global audience, through an open access platform. As an open access journal, Plastic and Reconstructive Surgery—Global Open offers its content for free to any viewer. Authors of articles retain their copyright to the materials published. Additionally, Plastic and Reconstructive Surgery—Global Open provides rapid review and publication of accepted papers.