Assessing microplastic and nanoplastic contamination in bird lungs: evidence of ecological risks and bioindicator potential

Abstract

Microplastics (MPs, 1 µm–5 mm) and nanoplastics (NPs, < 1 µm), collectively termed micro(nano)plastics (MNPs), are pervasive airborne pollutants with significant ecological risks. Birds, recognized as bioindicators, are particularly vulnerable to MNP exposure, yet the extent and risks of MNP pollution in bird lungs remain largely unexplored. This study assessed MP exposure in bird lungs of 51 species and NP exposure in the lungs of five representative species using laser direct infrared (LDIR) and pyrolysis gas chromatography-mass spectrometry (Py-GC-MS) techniques, respectively. The LDIR analysis revealed different degrees of MP contamination in bird lungs, with an average abundance of 221.20 items per species and 416.22 MP particles per gram of lung. Among 32 identified MP types, chlorinated polyethylene (CPE) and butadiene rubber (BR) predominated, with particles primarily in film and pellet forms, concentrated in the 20–50 μm size range. The polymer hazard index (PHI) indicated elevated ecological risks (levels Ⅲ or Ⅳ) in most bird lungs. Py-GC-MS detected nylon 66 (PA66), polyvinyl chloride (PVC), and polypropylene (PP) NPs at varying concentrations. Terrestrial, carnivorous, and larger-bodied birds exhibited higher MNP burdens. This study provides the first evidence of MNP contamination in bird lungs, highlighting their potential as bioindicators of airborne MNP pollution.