Fecal microbiota transplantation combined with inulin promotes the development and function of early immune organs in chicks.

IF 4.1 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of biotechnology Pub Date : 2025-01-16 DOI:10.1016/j.jbiotec.2025.01.012
Yang Song, Yibo Cui, Yue Zhong, Yumeng Wang, Xin Zheng
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Abstract

Modern management of chicks hinders the vertical transmission of intestinal microbiota, which is closely related to immunity. Inulin is a substrate that can be utilized by the microbiota. This study aimed to determine whether fecal microbiota transplantation (FMT) combined with inulin played a "1 + 1 > 2" role in enhancing the development and function of immune organs. Chicks were treated with 1 % inulin and/or fecal microbiota suspension on days 1-6. The growth performance, immune organ development, and immune indicators were evaluated on days 7, 14, and 21. Results showed that the combination of FMT and inulin significantly increased the immune organ index on day 7 and promoted the morphological structure and the expression of proliferating cell nuclear antigen (PCNA) in immune organs on days 7, 14, and 21. Each treatment increased the gene expression of interferon-gamma (IFN-γ), interleukin-4 (IL-4), interleukin-2 (IL-2), B cell-activating factor receptor (BAFFR), B cell linker (BLNK), C-X-C Motif Chemokine Ligand 12 (CXCL12), C-X-C Motif Chemokine Receptor 4 (CXCR4), and Biotin (Bu-1) to varying degrees. FMT combined with inulin significantly increased the expression of IgA-positive cells on days 7 and 14. In conclusion, the synergistic effect of FMT and inulin had beneficial impacts on the development and function of immune organs.

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粪菌群移植联合菊粉可促进雏鸡早期免疫器官的发育和功能。
雏鸡的现代管理阻碍了肠道菌群的垂直传播,而肠道菌群与免疫力密切相关。菊粉是一种可以被微生物利用的底物。本研究旨在确定粪便微生物群移植(FMT)联合菊粉是否在促进免疫器官发育和功能方面起到“1+1>2”的作用。雏鸡在第1-6天饲喂1%菊粉和/或粪便菌群悬浮液。分别于第7、14、21天评价生长性能、免疫器官发育及免疫指标。结果表明,FMT与菊粉联合用药可显著提高第7天免疫器官指数,促进第7、14、21天免疫器官形态结构和增殖细胞核抗原(PCNA)的表达。各处理均不同程度地增加干扰素γ (IFN-γ)、白介素-4 (IL-4)、白介素-2 (IL-2)、B细胞活化因子受体(BAFFR)、B细胞连接体(BLNK)、C-X-C基序趋化因子配体12 (CXCL12)、C-X-C基序趋化因子受体4 (CXCR4)和生物素(Bu-1)的基因表达。FMT联合菊粉可显著提高第7天和第14天iga阳性细胞的表达。综上所述,FMT和菊粉的协同作用对免疫器官的发育和功能有有益的影响。
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来源期刊
Journal of biotechnology
Journal of biotechnology 工程技术-生物工程与应用微生物
CiteScore
8.90
自引率
2.40%
发文量
190
审稿时长
45 days
期刊介绍: The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome.
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