Investigation of protein structure and quality of Acipenser sinensis frozen by liquid nitrogen.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2025-01-20 DOI:10.1002/jsfa.14115

Ling Tan, Jiwang Chen, Zihan Li, E Liao, Youling Xiong, Hongyan Lu

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Abstract

Background: The quality of Acipenser sinensis deteriorates significantly during frozen storage due to its high water, protein, and unsaturated fatty acid content. Conventional freezing methods are insufficient to preserve it effectively. This study investigated the effects of liquid nitrogen freezing (LNF) on the quality and myofibrillar protein (MP) characteristics of A. sinensis during frozen storage.

Results: Freezing A. sinensis with LNF resulted in faster freezing, lower free water content, smaller ice crystals, and less structural damage to MPs than refrigerator freezing (RF). It also resulted in higher shear force, Ca²⁺-ATPase activity, and total sulfhydryl content, as well as lower total volatile basic nitrogen (TVBN) values, thawing loss rate, and disulfide bond content, demonstrating that LNF can inhibit protein denaturation and delay the deterioration in the quality of A. sinensis during frozen storage. There were no significant differences between LNF-80 and LNF-110 in quality and myofibrillar protein characteristics of A. sinensis.

Conclusions: Liquid nitrogen freezing can maintain the quality of A. sinensis and reduce the degree of MP denaturation and oxidation during frozen storage. Freezing with LNF at -80 °C might be more appropriate for A. sinensis. The study has important implications for the application of cryogenic storage technology to aquatic products. © 2025 Society of Chemical Industry.

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液氮冷冻中华鲟鱼蛋白质结构及品质的研究。

背景：由于中华鲟鱼的水分、蛋白质和不饱和脂肪酸含量高，在冷冻储存期间，其品质会显著下降。传统的冷冻方法不足以有效保存。本研究研究了液氮冷冻对冬青品质和肌纤维蛋白（MP）特性的影响。结果：与冰箱冷冻（RF）相比，低温冷冻（lf）可使中华按蚊冷冻速度更快，游离水含量更低，冰晶更小，对MPs的结构损伤更小。结果表明，低温处理能显著提高冬青树的剪切力、Ca2+- atp酶活性和总巯基含量，降低总挥发性碱性氮（TVBN）值、解冻损耗率和二硫键含量，从而抑制冬青树冻存过程中蛋白质的变性，延缓冬青树品质的劣化。LNF-80和LNF-110在中华沙棘的品质和肌纤维蛋白特性上无显著差异。结论：液氮冷冻可以保持中华按蚊的品质，降低MP在冷冻贮藏过程中的变性和氧化程度。冬虫夏草在-80°C低温下低温冷冻较为适宜。该研究对水产品低温贮藏技术的应用具有重要意义。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.