Synergistic immunomodulatory effect of wheat/soybean/sea cucumber peptides in chloramphenicol induced immunosuppression zebrafish.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2025-03-10 DOI:10.1002/jsfa.14130

Qianqian Wang, Yiyang Xie, Junhui Zhang, Minjie Zhao, Jing Wang, Juan Du, Weihong Min, Fengqin Feng, Fei Shen

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Abstract

Background: Food-derived immunomodulatory peptides serve as vital agents in promoting health by protecting the body against pathogens. The present study focused on determining the optimal ratio for combining different peptides to form a complex that enhances immune activity in immunosuppressed zebrafish.

Results: We established a method for immunosuppression in zebrafish, discovering that treatment with 125 μg mL^-1 chloramphenicol significantly decreased the macrophage number and neutrophil fluorescence intensity. We then assessed the immunomodulatory activity of soft-shelled turtle peptides, sea cucumber peptides (SCP), black-bone silky fowl peptides, soybean peptides (SP), wheat peptides (WP), whey protein peptides and casein hydrolysate peptides. The results of individual peptides showed that WP uniquely increased the interferon-γ level, SP most effectively increased neutrophil fluorescence intensity, and SCP increased both macrophage number and neutrophil fluorescence intensity. Using mixture design and fuzzy comprehensive evaluation methods, we identified a synergistic mixture peptides: 8.32% SCP + 20.84% SP + 70.84% WP, which had the highest score in the fuzzy comprehensive evaluation, making it the best blend for boosting zebrafish immunity.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.