Synergistic immunomodulatory effect of wheat/soybean/sea cucumber peptides in chloramphenicol induced immunosuppression zebrafish

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2025-03-10 DOI:10.1002/jsfa.14130

Qianqian Wang, Yiyang Xie, Junhui Zhang, Minjie Zhao, Jing Wang, Juan Du, Weihong Min, Fengqin Feng, Fei Shen

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Abstract

BACKGROUND

Food-derived immunomodulatory peptides serve as vital agents in promoting health by protecting the body against pathogens. The present study focused on determining the optimal ratio for combining different peptides to form a complex that enhances immune activity in immunosuppressed zebrafish.

RESULTS

We established a method for immunosuppression in zebrafish, discovering that treatment with 125 μg mL⁻¹ chloramphenicol significantly decreased the macrophage number and neutrophil fluorescence intensity. We then assessed the immunomodulatory activity of soft-shelled turtle peptides, sea cucumber peptides (SCP), black-bone silky fowl peptides, soybean peptides (SP), wheat peptides (WP), whey protein peptides and casein hydrolysate peptides. The results of individual peptides showed that WP uniquely increased the interferon-γ level, SP most effectively increased neutrophil fluorescence intensity, and SCP increased both macrophage number and neutrophil fluorescence intensity. Using mixture design and fuzzy comprehensive evaluation methods, we identified a synergistic mixture peptides: 8.32% SCP + 20.84% SP + 70.84% WP, which had the highest score in the fuzzy comprehensive evaluation, making it the best blend for boosting zebrafish immunity.

CONCLUSION

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小麦/大豆/海参多肽对氯霉素诱导免疫抑制斑马鱼的协同免疫调节作用。

背景：食物来源的免疫调节肽通过保护身体免受病原体的侵害，在促进健康方面发挥着重要作用。本研究的重点是确定组合不同肽形成复合物的最佳比例，以增强免疫抑制斑马鱼的免疫活性。结果：我们建立了斑马鱼免疫抑制方法，发现125 μg mL-1氯霉素可显著降低巨噬细胞数量和中性粒细胞荧光强度。研究了甲鱼肽、海参肽、乌骨鸡肽、大豆肽、小麦肽、乳清蛋白肽和酪蛋白水解肽的免疫调节活性。单肽实验结果显示，WP能惟一提高干扰素-γ水平，SP能最有效地提高中性粒细胞荧光强度，SCP能同时提高巨噬细胞数量和中性粒细胞荧光强度。采用混合设计和模糊综合评价方法，确定了8.32% SCP + 20.84% SP + 70.84% WP的协同多肽组合，模糊综合评价得分最高，是提高斑马鱼免疫力的最佳组合。结论：一种特殊的多肽组合（8.32% SCP + 20.84% SP + 70.84% WP）具有协同免疫调节作用。©2025化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.