Quercetin improves heart failure by inhibiting cardiomyocyte apoptosis via suppressing the MAPK signaling pathway.

Xiupeng Long, Shun Tao, Shen Yang, Suyun Li, Libing Rao, Li Li, Zhe Zhang
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Abstract

Objectives: To explore the mechanism that mediate the therapeutic effect of quercetin on heart failure.

Methods: We searched the TCMSP and Swiss ADME databases for the therapeutic targets of quercetin and retrieved heart failure targets from the Genecards and OMIM databases. The intersecting targets were analyzed with GO and KEGG pathway analysis using DAVID database, and the key genes were identified via PPI analysis. Molecular docking between the core targets and quercetin was performed using PyMOL and AutoDock Tools. In a heart failure model established in H9C2 cardiomyocytes by treatment with isoproterenol, the effect of quercetin on the expressions of the MAPK signaling pathway was tested.

Results: A total of 60 intersecting targets were identified. Enrichment analysis revealed that quercetin may inhibit heart failure through the MAPK signaling pathway. The core genes, including AMPK3 and BCL-2, were identified as potential key regulators in quercetin-mediated improvement of heart failure. Cellular experiments demonstrated that quercetin significantly reduced isoproterenol-induced apoptosis of cardiomyocytes in a dose-dependent manner and obviously decreased the Bax/Bcl-2 ratio and the expression levels of caspase-3, ERK and p38 in the cells.

Conclusions: Quercetin improves heart failure possibly by inhibiting cardiomyocyte apoptosis through the MAPK signaling pathway.

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槲皮素通过抑制MAPK信号通路抑制心肌细胞凋亡改善心力衰竭。
目的:探讨槲皮素治疗心力衰竭的作用机制。方法:在TCMSP和Swiss ADME数据库中检索槲皮素的治疗靶点,在Genecards和OMIM数据库中检索心衰靶点。使用DAVID数据库对交叉靶点进行GO和KEGG通路分析,通过PPI分析鉴定关键基因。利用PyMOL和AutoDock Tools进行核心靶点与槲皮素的分子对接。通过异丙肾上腺素处理H9C2心肌细胞建立心力衰竭模型,检测槲皮素对MAPK信号通路表达的影响。结果:共鉴定出60个相交靶点。富集分析显示槲皮素可能通过MAPK信号通路抑制心力衰竭。核心基因,包括AMPK3和BCL-2,被确定为槲皮素介导的心力衰竭改善的潜在关键调节因子。细胞实验表明,槲皮素显著降低异丙肾上腺素诱导的心肌细胞凋亡,并呈剂量依赖性,显著降低细胞中Bax/Bcl-2比值及caspase-3、ERK、p38的表达水平。结论:槲皮素可能通过MAPK信号通路抑制心肌细胞凋亡,从而改善心力衰竭。
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来源期刊
南方医科大学学报杂志
南方医科大学学报杂志 Medicine-Medicine (all)
CiteScore
1.50
自引率
0.00%
发文量
208
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