Identification of the bHLH gene family and functional analysis of ChMYC2 in drought stress of Cerasus humilis

IF 5.7 2区生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2025-03-01 Epub Date: 2025-01-18 DOI:10.1016/j.plaphy.2025.109519

Buming Dong , Shaoyu Lang , Yongmei Gu , Xin Liu , Xingshun Song

{"title":"Identification of the bHLH gene family and functional analysis of ChMYC2 in drought stress of Cerasus humilis","authors":"Buming Dong , Shaoyu Lang , Yongmei Gu , Xin Liu , Xingshun Song","doi":"10.1016/j.plaphy.2025.109519","DOIUrl":null,"url":null,"abstract":"<div><div>The basic helix-loop-helix (bHLH) transcription factors (TFs) play a crucial regulatory role in the growth and development of plants, as well as in their response to environmental stresses. In this study, we identified 94 ChbHLHs from Cerasus humilis, an economically valuable tree native to northern China. We analyzed their evolutionary relationships, gene structures, chromosome distributions, promoter cis-regulatory elements, and collinearity. Our analysis revealed numerous cis-regulatory elements associated with phytohormone responses and abiotic stress within the upstream promoter sequences of ChbHLH genes. The transcriptome results indicated that 84 ChbHLHs exhibited differential expression under drought conditions. Among those with upregulated expression levels, we selected ChMYC2 (ChbHLH93) for further investigation. Overexpressing ChMYC2 in Arabidopsis thaliana led to significantly elevated expression of drought-responsive genes compared to wild-type (WT) plants, resulting in enhanced drought resistance. Furthermore, we identified a gene, ChABI5 (ABA-insensitive 5), which interacts with ChMYC2. This study provides valuable genetic resources for future cultivation efforts aimed at developing stress-resistant and economically viable trees.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"Article 109519"},"PeriodicalIF":5.7000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825000476","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/18 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

The basic helix-loop-helix (bHLH) transcription factors (TFs) play a crucial regulatory role in the growth and development of plants, as well as in their response to environmental stresses. In this study, we identified 94 ChbHLHs from Cerasus humilis, an economically valuable tree native to northern China. We analyzed their evolutionary relationships, gene structures, chromosome distributions, promoter cis-regulatory elements, and collinearity. Our analysis revealed numerous cis-regulatory elements associated with phytohormone responses and abiotic stress within the upstream promoter sequences of ChbHLH genes. The transcriptome results indicated that 84 ChbHLHs exhibited differential expression under drought conditions. Among those with upregulated expression levels, we selected ChMYC2 (ChbHLH93) for further investigation. Overexpressing ChMYC2 in Arabidopsis thaliana led to significantly elevated expression of drought-responsive genes compared to wild-type (WT) plants, resulting in enhanced drought resistance. Furthermore, we identified a gene, ChABI5 (ABA-insensitive 5), which interacts with ChMYC2. This study provides valuable genetic resources for future cultivation efforts aimed at developing stress-resistant and economically viable trees.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

华北樱bHLH基因家族鉴定及ChMYC2基因在干旱胁迫下的功能分析。

基本螺旋-环-螺旋（bHLH）转录因子（TFs）在植物的生长发育以及对环境胁迫的响应中起着至关重要的调节作用。在这项研究中，我们从原产于中国北方的一种具有经济价值的树木——矮樱桃中鉴定出94个ChbHLHs。我们分析了它们的进化关系、基因结构、染色体分布、启动子顺式调控元件和共线性。我们的分析揭示了ChbHLH基因上游启动子序列中与植物激素反应和非生物胁迫相关的许多顺式调控元件。转录组结果表明，84个ChbHLHs在干旱条件下表现出差异表达。在表达水平上调的基因中，我们选择了ChMYC2 （ChbHLH93）进行进一步的研究。与野生型（WT）植物相比，拟南芥过表达ChMYC2导致干旱响应基因的表达显著升高，从而增强了其抗旱性。此外，我们还发现了一个与ChMYC2相互作用的基因ChABI5 （ABA-insensitive 5）。该研究为今后培育抗逆性强、经济上可行的树木提供了宝贵的遗传资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.