ZmSIDP1, a DUF1644 gene from the halophyte Zoysia matrella, positively regulates salt tolerance in rice.

Abstract

As a detrimental abiotic stressor, salinity affects plant growth and yield. Domain of unknown function 1644 (DUF1644) is a large plant-specific DUF protein family that is predicted to be involved in abiotic stress responses in plants. However, the biological functions of DUF1644 genes in plants remain largely unexplored, especially in halophytes. Here, we investigated the function of the DUF1644 gene, ZmSIDP1, from the halophyte Zoysia matrella. ZmSIDP1 could enhance the salt tolerance of yeast. Furthermore, the heterologous transformation of the ZmSIDP1 gene in rice demonstrated that transgenic rice plants exhibited better growth under NaCl treatment. The Na ⁺ content was lower in ZmSIDP1 transgenic rice than in wild-type rice under salt stress. ZmSIDP1 transgenic rice showed stronger resistance to oxidative stress induced by salt stress. Further investigation indicated that ZmSIDP1 could interact with an HD-Zip transcription factor, ZmROC1. These results suggest that the ZmSIDP1 gene from the halophyte Z. matrella can positively regulate salt resistance in rice, laying a foundation for the application of salt tolerance genes from halophytes to enhance salt tolerance in rice.