Production and application of Paenibacillus polymyxa levan as an antiviral priming agent against cucumber mosaic virus infection in tobacco plants

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED Carbohydrate Polymers Pub Date : 2025-03-15 Epub Date: 2024-12-31 DOI:10.1016/j.carbpol.2024.123208

Hyunjun Ko , Hangil Kim , Ji Won Heo , Sangjun Lee , Yongtae Yu , Hyebin Kim , Hyeon-Seok Oh , Chan-Gi Min , Seokmin Baek , Han Suk Choi , Sung Jin Moon , Woohyun Sim , Byung Sup Kim , Jin-sung Hong , Sangrea Shim , Yong Sik Kim

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Abstract

This study investigates the application of levan- produced from Paenibacillus polymyxa SG09–12 as an antiviral agent against cucumber mosaic virus (CMV). A high-purity microbial levan was produced and purified using diafiltration. The chemical composition, structure, and functional groups of the levan were characterised using high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS). Antiviral effects of the levan were evaluated in Nicotiana tabacum plants infected with CMV. Treatment with the purified levan significantly attenuated viral symptoms and reduced viral titres, demonstrating a remarkable, long-lasting antiviral effect and highlighting its potential as an antiviral agent. This antiviral effect may have been mediated by transcriptional activation of disease resistance genes encoding RPP13. These findings enhance the understanding of levan produced by Paenibacillus species and their application as an antiviral defense mechanism, which may contribute towards sustainable and environmentally friendly crop protection strategies.

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多粘类芽孢杆菌levan在烟草上黄瓜花叶病毒抗病毒引物的制备及应用。

研究了多粘类芽孢杆菌SG09-12产的利末酸作为黄瓜花叶病毒（CMV）的抗病毒药物的应用。制备了一种高纯度的微生物利凡，并采用滤除法提纯。采用高效液相色谱（HPLC）、核磁共振（NMR）、傅里叶变换红外光谱（FT-IR）和x射线光电子能谱（XPS）等方法对其化学成分、结构和官能团进行了表征。研究了利凡碱对感染巨细胞病毒的烟草植株的抗病毒作用。用纯化的利凡碱治疗可显著减轻病毒症状并降低病毒滴度，显示出显著的、持久的抗病毒效果，并突出了其作为抗病毒药物的潜力。这种抗病毒作用可能是通过编码RPP13的抗病基因的转录激活介导的。这些发现加深了对芽孢杆菌产生的利末酸及其作为抗病毒防御机制的认识，可能有助于制定可持续和环境友好型作物保护策略。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.