Production and application of Paenibacillus polymyxa levan as an antiviral priming agent against cucumber mosaic virus infection in tobacco plants.

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED Carbohydrate Polymers Pub Date : 2025-03-15 Epub Date: 2024-12-31 DOI:10.1016/j.carbpol.2024.123208

Hyunjun Ko, Hangil Kim, Ji Won Heo, Sangjun Lee, Yongtae Yu, Hyebin Kim, Hyeon-Seok Oh, Chan-Gi Min, Seokmin Baek, Han Suk Choi, Sung Jin Moon, Woohyun Sim, Byung Sup Kim, Jin-Sung Hong, Sangrea Shim, Yong Sik Kim

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引用次数: 0

Abstract

This study investigates the application of levan- produced from Paenibacillus polymyxa SG09-12 as an antiviral agent against cucumber mosaic virus (CMV). A high-purity microbial levan was produced and purified using diafiltration. The chemical composition, structure, and functional groups of the levan were characterised using high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS). Antiviral effects of the levan were evaluated in Nicotiana tabacum plants infected with CMV. Treatment with the purified levan significantly attenuated viral symptoms and reduced viral titres, demonstrating a remarkable, long-lasting antiviral effect and highlighting its potential as an antiviral agent. This antiviral effect may have been mediated by transcriptional activation of disease resistance genes encoding RPP13. These findings enhance the understanding of levan produced by Paenibacillus species and their application as an antiviral defense mechanism, which may contribute towards sustainable and environmentally friendly crop protection strategies.

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.