Therapeutic efficacy of intra-articular injection of human adipose-derived mesenchymal stem cells in a sheep model of knee osteoarthritis.

IF 7.3 2区医学 Q1 CELL & TISSUE ENGINEERING Stem Cell Research & Therapy Pub Date : 2025-01-23 DOI:10.1186/s13287-025-04143-6

Jigang Lei, Xingyi Chen, Haohao Xie, Yuhao Dai, Zhongjin Chen, Liang Xu

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Abstract

Background: Mesenchymal stem cells have great potential for repairing articular cartilage and treating knee osteoarthritis (KOA). Nonetheless, little is known about the efficacy of human adipose-derived mesenchymal stem cells (haMSCs) for KOA in large animal models.

Methods: This study evaluated the therapeutic efficacy of haMSCs in knee articular cartilage repair in a sheep model of KOA. haMSCs were isolated, cultured, and characterized. KOA was surgically induced by anterior cruciate ligament transection and medial meniscectomy, followed by intra-articular injection of saline (negative control group) or haMSCs (haMSC group) into the right knee joint at 6 and 9 weeks after surgery. Sheep were sacrificed 21 weeks after surgery, and samples (whole knee joints, femoral condyles, and tibias) were collected, processed, and analyzed. Changes in knee articular cartilage were assessed by magnetic resonance imaging, micro-computed tomography, macroscopic analysis, histology, and immunohistochemistry.

Results: KOA caused the degeneration of the medial femoral condyle in the sheep model of KOA. Conversely, haMSCs repaired chondral defects and increased the thickness of knee articular cartilage.

Conclusions: These data suggest that the intra-articular injection of haMSCs can effectively repair articular cartilage defects in the knee.

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人脂肪源性间充质干细胞关节内注射对羊膝骨关节炎模型的治疗效果。

背景：间充质干细胞在修复关节软骨和治疗膝骨关节炎（KOA）方面具有巨大的潜力。然而，在大型动物模型中，人类脂肪来源的间充质干细胞（haMSCs）对KOA的疗效知之甚少。方法：本研究评价haMSCs对KOA羊模型膝关节软骨修复的治疗效果。分离、培养haMSCs并对其进行表征。手术诱导KOA的方法为前交叉韧带横断和内侧半月板切除术，术后6周和9周分别向右膝关节关节内注射生理盐水（阴性对照组）或haMSC （haMSC组）。手术后21周处死羊，收集样本（整个膝关节、股骨髁和胫骨），进行处理和分析。通过磁共振成像、显微计算机断层扫描、宏观分析、组织学和免疫组织化学评估膝关节软骨的变化。结果：KOA引起羊KOA模型股骨内侧髁退变。相反，haMSCs修复软骨缺损，增加膝关节软骨厚度。结论：关节内注射haMSCs可有效修复膝关节软骨缺损。

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来源期刊

Stem Cell Research & Therapy CELL BIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL

CiteScore

13.20

自引率

8.00%

发文量

525

审稿时长

1 months

期刊介绍： Stem Cell Research & Therapy serves as a leading platform for translational research in stem cell therapies. This international, peer-reviewed journal publishes high-quality open-access research articles, with a focus on basic, translational, and clinical research in stem cell therapeutics and regenerative therapies. Coverage includes animal models and clinical trials. Additionally, the journal offers reviews, viewpoints, commentaries, and reports.