The Effect of Lipopolysaccharides from Salmonella enterica on the Size, Density, and Compressibility of Phospholipid Vesicles.

IF 3.4 3区医学 Q1 ENGINEERING, MULTIDISCIPLINARY Biomimetics Pub Date : 2025-01-15 DOI:10.3390/biomimetics10010055

Tamás Szabó, Zuzana Garaiová, Sopio Melikishvili, Marek Tatarko, Zsófia Keresztes, Tibor Hianik

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Abstract

The properties of the large unilamellar vesicles (LUVs) from 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), modified by lipopolysaccharides (LPS) from Salmonella enterica sv. Enteritidis, which mimics Gram-negative bacteria, were studied by various physical methods. LPS, in the range of 0/20/50 % w/w relative to the lipid, had a regulatory role in the structure of the LUVs toward the lower size, low polydispersity, and over-a-month size stability due to the lower negative zeta potential. The addition of LPS resulted in increased density, which determined the ultrasound velocity and the specific adiabatic compressibility. In a 0.5/1/2 mg/mL concentration range, the total lipid content did not significantly affect the size of LUVs and influenced the density-related attributes similarly to the LPS content. A positive correlation was found between temperature and vesicle size, and a negative correlation was found between temperature and density and compressibility-except for the anomaly behavior at 25 °C, around the melting point of DMPC.

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