Effects of subclinical hypothyroidism during pregnancy on mtDNA methylation in the brain of rat offspring.

IF 2.3 4区医学 Q3 NEUROSCIENCES BMC Neuroscience Pub Date : 2025-01-24 DOI:10.1186/s12868-025-00930-2

Liangzhuo Xie, Yangling Huang, Xiande Ma, Xiaoqiu Ma, Jian Wang, Tianshu Gao, Wei Chen

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Abstract

Objective: This study aims to investigate the impact of subclinical hypothyroidism (SCH) during pregnancy on mitochondrial DNA (mtDNA) methylation in the brain tissues of rat offspring.

Materials and methods: Sixteen SD rats were randomly divided into two groups: control group (CON) and SCH group. BS-seq sequencing was used to analyze mtDNA methylation levels in the offspring's brain tissues; the 2,7-dichlorofluorescin diacetate (DCFH-DA) probe method was employed to detect reactive oxygen species (ROS) levels in brain tissues; electron microscopy was utilized to observe the mitochondrial structure in the hippocampal tissues of the offspring.

Results: In the analysis of differentially methylated regions (DMRs), the mitochondrial chromosome in the SCH group exhibited 23 DMRs compared to the control group. ROS levels in the brain tissues of the SCH group were significantly higher than those in the control group (P < 0.05). The mitochondrial structure in the hippocampus of the SCH group was less intact compared to the CON group.

Conclusion: Subclinical hypothyroidism in pregnant rats may alter the mtDNA methylation pattern in the brains of their offspring, potentially affecting mitochondrial function and structure.

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妊娠亚临床甲状腺功能减退症对大鼠后代脑mtDNA甲基化的影响。

目的：探讨妊娠期亚临床甲状腺功能减退症（SCH）对大鼠子代脑组织线粒体DNA （mtDNA）甲基化的影响。材料与方法：将16只SD大鼠随机分为对照组（CON）和SCH组。采用BS-seq测序分析后代脑组织中mtDNA甲基化水平；采用2,7-二氯荧光素（DCFH-DA）探针法检测脑组织活性氧（ROS）水平；电镜观察子代海马组织线粒体结构。结果：在差异甲基化区（DMRs）分析中，与对照组相比，SCH组线粒体染色体出现23个差异甲基化区。结论：妊娠大鼠亚临床甲状腺功能减退症可能改变其后代大脑mtDNA甲基化模式，可能影响线粒体功能和结构。

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来源期刊

BMC Neuroscience 医学-神经科学

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

16 months

期刊介绍： BMC Neuroscience is an open access, peer-reviewed journal that considers articles on all aspects of neuroscience, welcoming studies that provide insight into the molecular, cellular, developmental, genetic and genomic, systems, network, cognitive and behavioral aspects of nervous system function in both health and disease. Both experimental and theoretical studies are within scope, as are studies that describe methodological approaches to monitoring or manipulating nervous system function.