Crocin facilitates osteogenesis and angiogenesis by moderating oxidative stress and ferroptosis via Nrf2/GPX4 pathway

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2024-12-07 DOI:10.1016/j.tice.2024.102675

Ruilong Hong, Bo Chen, Hao Wu, Junwen Ding

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引用次数: 0

Abstract

Bone formation is a complex multi-factor process of bone defect healing. Oxidative stress (OS) is predisposed to induce regulatory cell death (RCD), such as ferroptosis. At present, the antioxidant effects of Crocin on erastin induced oxidative damage were studied. The activity of bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs) was detected by CCK-8 and EdU staining. The production of reactive oxygen species (ROS), MDA, SOD and GSH were evaluated. Western blotting assay was used to detect ferroptosis-related proteins. The osteogenic function of BMSCs was determined by alkaline phosphatase (ALP) activity, ALP staining and alizarin red S (ARS) staining. Western blotting and RT-PCR assays were used to detect the expression of osteogenic proteins and genes. Angiogenesis of HUVECs was evaluated by tube formation, RT-PCR, scratch test and Transwell assay. The results showed that Crocin can promote the osteogenic function of BMSCs and angiogenesis of HUVECs. In addition, Crocin protects cells from erastin-induced oxidative injury and inhibits ferroptosis via the Nrf2/GPX4 pathway. These findings suggest that Crocin can promote bone defect healing by regulating OS and inhibiting ferroptosis through the Nrf2/GPX4 pathway.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.