A high-performance aptasensing interface based on pseudo-AuNBs@Ti3C2Tx MXene nanocomposite for non-invasive measurement of carbamazepine in human biofluids

Abstract

For the first time, a novel aptasensing interface based on smart integration of pseudo-gold nanobons (AuNBs) and Ti₃C₂T_x MXene is introduced for high selective detection of carbamazepine (CBZ). The large specific surface area achieved from the proposed nanocomposite increases the targeted immobilization of the Apt sequence on the surface via AuNBs as the linkage. It embeds a high-performance grafting platform for trapping CBZ with high sensitivity and accuracy in human biofluids and pharmaceutical formulations. The molecular dynamic (MD) simulation method that exhibits how the Apt binds to CBZ in a conformation-switching assay format from a molecular view is a valid certification for the interaction of CBZ on the developed aptasensing interface. The aptasensor measured CBZ from 1 fM to 100 nM with a superior detection limit (LOD) value of 330 aM compared with other reported CBZ sensors. Due to using biocompatible and non-toxic compounds, consuming low energy and chemicals the greenness of the proposed strategy has been certified by the international scoring system.

Graphical Abstract