Effect of storage temperature on bovine cardiac morphology.

IF 1.4 3区农林科学 Q2 VETERINARY SCIENCES American journal of veterinary research Pub Date : 2025-01-30 Print Date: 2025-04-01 DOI:10.2460/ajvr.24.11.0329

Tara L Goertzen, Claire R Shaw, Lauren M Gilbertsen, Yufei Guo, Emma S Scudder, Phillip D Bass, Lauren E Christensen, Daniel P Fitzsimons

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引用次数: 0

Abstract

Objective: To determine the effects of 2 storage protocols on postmortem cardiac morphology.

Methods: 11 hearts were used and randomly assigned to 1 of 2 treatment groups: refrigeration for 7 days at 4 °C (group 1) or frozen for 6 days at -20 °C (group 2). Hearts were evaluated for weight, ventricular and intraventricular septal wall thickness, and valvular thickness circumference. The hearts in group 1 were measured daily for 7 days, whereas the hearts in group 2 were measured on day 0, frozen for 6 days, and thawed at ambient temperature overnight and remeasured on day 7.

Results: The effect of day had a significant impact on left ventricular (LV) apex thickness for both groups 1 and 2. The LV apex thickness was reduced by approximately 1.9 mm (group 1) and 0.8 mm (group 2). Day also had a significant effect on LV midwall in group 1; however, it was not significant until day 6, indicating a longer period of tissue stability compared to LV apex.

Conclusions: The LV apex was the first parameter to change; however, it is important to take into consideration that the majority of the parameters did not change significantly when determining a timeline. This study found refrigeration as a viable option for short-term storage depending on collection timeframe.

Clinical relevance: This study is clinically relevant as it gives veterinarians time to assess necropsied tissue.

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贮藏温度对牛心脏形态的影响。

目的：探讨两种储存方式对死后心脏形态的影响。方法：11颗心脏随机分为2个治疗组：在4°C冷藏7天（1组）或在-20°C冷冻6天（2组）。评估心脏的重量、心室和室间隔壁厚度以及瓣膜厚度周长。第1组连续7天每天测量心脏，第2组于第0天测量心脏，冷冻6天，室温解冻过夜，第7天再次测量心脏。结果：日效对1、2组左室心尖厚度均有显著影响。左室尖部厚度分别减少1.9 mm（组1）和0.8 mm（组2）。Day对左室中壁的影响也很显著；然而，直到第6天才显著，这表明与左心室尖相比，左心室尖的组织稳定性更长。结论：左室心尖是最先发生变化的参数；然而，重要的是要考虑到，在确定时间表时，大多数参数没有显著变化。这项研究发现，根据收集时间，冷藏是短期储存的可行选择。临床相关性：这项研究具有临床相关性，因为它给兽医时间来评估坏死组织。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

10.00%

发文量

186

审稿时长

3 months

期刊介绍： The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.