Electroacupuncture promotes neural function recovery by alleviating mitochondria damage in cerebral ischemia mice

IF 2.6 4区医学 Q3 NEUROSCIENCES Brain Research Pub Date : 2025-03-15 Epub Date: 2025-01-30 DOI:10.1016/j.brainres.2025.149479

Feng Yashuo , Guan Chong , Yang Zhe , Cao Lu , Xie Hongyu , Wu Yi , Wang Nianhong

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Abstract

Aims

This study aimed to observe the effect of electroacupuncture (EA) at Zusanli point (ST36) on motor function of cerebral ischemia mice, and to observe the effect of EA on mitochondrial morphology of peri-infarct cortex neurons in cerebral ischemia mice.

Methods

Middle cerebral artery occlusion (MCAO) was used to develop an ischemic stroke mice model. EA treatment was performed for three consecutive days for 15 min per day after MCAO modeling. We investigated the therapeutic effects of EA on MCAO mice by performing neurobehavioral assessment (modified Neurological Severity Score, Rotarod test, Open-field test and Gait analysis) and TTC staining. The morphology and function of neuronal mitochondria were evaluated by transmission electron microscopy, qRT-PCR, chemiluminescence, and western blot. Nissl staining, TUNEL staining and immunofluorescence staining were used to observe neuronal morphology and apoptosis. Furthermore, ELISA was employed to measure the expression levels of inflammatory factors in mouse serum.

Results

EA alleviated motor dysfunction and infarct volume in mice with cerebral ischemia. It improved the neuronal mitochondria damage in MCAO mice, and decreased the protein and mRNA expression level of mitochondrial fission related proteins (FIS1 and Drp1). In addition, EA can reduce neuronal damage and apoptosis of nerve cells, and decrease the level of inflammatory factors (IL-1β, TNF-α, IL-6 and IL-8) in cerebral ischemia mice.

Conclusion

EA therapy can improve motor dysfunction and alleviate the damage of neuron mitochondria in cerebral ischemic mice.

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电针通过减轻脑缺血小鼠线粒体损伤促进神经功能恢复。

目的：观察电针足三里穴（ST36）对脑缺血小鼠运动功能的影响，并观察电针对脑缺血小鼠梗死周围皮层神经元线粒体形态的影响。方法：采用大脑中动脉闭塞法（MCAO）建立小鼠缺血性脑卒中模型。MCAO造模后，连续3天给予EA治疗，每天15 min。我们通过神经行为评估（改良神经严重程度评分、Rotarod试验、Open-field试验和步态分析）和TTC染色研究EA对MCAO小鼠的治疗作用。采用透射电镜、qRT-PCR、化学发光、western blot等方法观察神经元线粒体的形态和功能。采用尼氏染色、TUNEL染色及免疫荧光染色观察神经元形态及凋亡情况。采用ELISA法检测小鼠血清中炎症因子的表达水平。结果：EA可减轻脑缺血小鼠的运动功能障碍和梗死面积。改善MCAO小鼠神经元线粒体损伤，降低线粒体裂变相关蛋白（FIS1和Drp1）的蛋白和mRNA表达水平。此外，EA可减轻脑缺血小鼠的神经元损伤和神经细胞凋亡，降低炎症因子（IL-1β、TNF-α、IL-6、IL-8）水平。结论：EA治疗可改善脑缺血小鼠运动功能障碍，减轻神经元线粒体损伤。

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来源期刊

Brain Research 医学-神经科学

CiteScore

5.90

自引率

3.40%

发文量

268

审稿时长

47 days

期刊介绍： An international multidisciplinary journal devoted to fundamental research in the brain sciences. Brain Research publishes papers reporting interdisciplinary investigations of nervous system structure and function that are of general interest to the international community of neuroscientists. As is evident from the journals name, its scope is broad, ranging from cellular and molecular studies through systems neuroscience, cognition and disease. Invited reviews are also published; suggestions for and inquiries about potential reviews are welcomed. With the appearance of the final issue of the 2011 subscription, Vol. 67/1-2 (24 June 2011), Brain Research Reviews has ceased publication as a distinct journal separate from Brain Research. Review articles accepted for Brain Research are now published in that journal.