Cascade signal amplification colorimetric aptasensor based on DNAzyme-driven DNA walker and catalytic hairpin assembly for the detection of aflatoxin B1

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Food Control Pub Date : 2025-01-06 DOI:10.1016/j.foodcont.2024.111124

Qiqi Wu , Yunzhe Zhang , Xin Lu , Congyan Qi , Xiaoyan Ma , Wei Zhang

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引用次数: 0

Abstract

Aflatoxin B₁ is one of the most toxic mycotoxin metabolites, which seriously endangers human health. Therefore, it is crucial to develop rapid, sensitive and reliable methods for AFB₁ detection. Herein, we proposed an enzyme-free cascade signal amplification colorimetric aptasensor based on DNA walker and catalytic hairpin assembly reaction (CHA), which was capable of visual qualitative detection and quantitative detection of AFB₁ in food products. Under the optimized experimental parameters, the proposed aptasensor visualised a qualitative detection limit of 1.5 ng/mL and a quantitative detection limit of 9.33 pg/mL, and sensitively detected AFB₁ in the linear range of 10 pg/mL-50 ng/mL. In addition, the aptasensor was successfully applied to the detection of AFB₁ in food, and the results were more consistent with those of ID-LC-MS/MS. The cascade signal amplification strategy provides a new platform for the rapid and sensitive detection of AFB₁.

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索莱宝

N,N,N’,N’-Tetramethylenediamine (TEMED)

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30% acrylamide solution

来源期刊

Food Control 工程技术-食品科技

CiteScore

12.20

自引率

6.70%

发文量

758

审稿时长

33 days

期刊介绍： Food Control is an international journal that provides essential information for those involved in food safety and process control. Food Control covers the below areas that relate to food process control or to food safety of human foods: • Microbial food safety and antimicrobial systems • Mycotoxins • Hazard analysis, HACCP and food safety objectives • Risk assessment, including microbial and chemical hazards • Quality assurance • Good manufacturing practices • Food process systems design and control • Food Packaging technology and materials in contact with foods • Rapid methods of analysis and detection, including sensor technology • Codes of practice, legislation and international harmonization • Consumer issues • Education, training and research needs. The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.