Cascade signal amplification colorimetric aptasensor based on DNAzyme-driven DNA walker and catalytic hairpin assembly for the detection of aflatoxin B1

IF 6.3 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Food Control Pub Date : 2025-05-01 Epub Date: 2025-01-06 DOI:10.1016/j.foodcont.2024.111124

Qiqi Wu , Yunzhe Zhang , Xin Lu , Congyan Qi , Xiaoyan Ma , Wei Zhang

{"title":"Cascade signal amplification colorimetric aptasensor based on DNAzyme-driven DNA walker and catalytic hairpin assembly for the detection of aflatoxin B1","authors":"Qiqi Wu , Yunzhe Zhang , Xin Lu , Congyan Qi , Xiaoyan Ma , Wei Zhang","doi":"10.1016/j.foodcont.2024.111124","DOIUrl":null,"url":null,"abstract":"<div><div>Aflatoxin B<sub>1</sub> is one of the most toxic mycotoxin metabolites, which seriously endangers human health. Therefore, it is crucial to develop rapid, sensitive and reliable methods for AFB<sub>1</sub> detection. Herein, we proposed an enzyme-free cascade signal amplification colorimetric aptasensor based on DNA walker and catalytic hairpin assembly reaction (CHA), which was capable of visual qualitative detection and quantitative detection of AFB<sub>1</sub> in food products. Under the optimized experimental parameters, the proposed aptasensor visualised a qualitative detection limit of 1.5 ng/mL and a quantitative detection limit of 9.33 pg/mL, and sensitively detected AFB<sub>1</sub> in the linear range of 10 pg/mL-50 ng/mL. In addition, the aptasensor was successfully applied to the detection of AFB<sub>1</sub> in food, and the results were more consistent with those of ID-LC-MS/MS. The cascade signal amplification strategy provides a new platform for the rapid and sensitive detection of AFB<sub>1</sub>.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"171 ","pages":"Article 111124"},"PeriodicalIF":6.3000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Control","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956713524008417","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/6 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Aflatoxin B₁ is one of the most toxic mycotoxin metabolites, which seriously endangers human health. Therefore, it is crucial to develop rapid, sensitive and reliable methods for AFB₁ detection. Herein, we proposed an enzyme-free cascade signal amplification colorimetric aptasensor based on DNA walker and catalytic hairpin assembly reaction (CHA), which was capable of visual qualitative detection and quantitative detection of AFB₁ in food products. Under the optimized experimental parameters, the proposed aptasensor visualised a qualitative detection limit of 1.5 ng/mL and a quantitative detection limit of 9.33 pg/mL, and sensitively detected AFB₁ in the linear range of 10 pg/mL-50 ng/mL. In addition, the aptasensor was successfully applied to the detection of AFB₁ in food, and the results were more consistent with those of ID-LC-MS/MS. The cascade signal amplification strategy provides a new platform for the rapid and sensitive detection of AFB₁.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

基于dnazyme驱动的DNA助行器和催化发夹组件的级联信号放大比色传感器检测黄曲霉毒素B1

黄曲霉毒素B1是毒性最强的真菌毒素代谢物之一，严重危害人体健康。因此，建立快速、灵敏、可靠的AFB1检测方法至关重要。本研究提出了一种基于DNA助行器和催化发夹组装反应（CHA）的无酶级联信号放大比色传感器，能够对食品中AFB1进行视觉定性和定量检测。在优化的实验参数下，该传感器的定性检出限为1.5 ng/mL，定量检出限为9.33 pg/mL，在10 pg/mL ~ 50 ng/mL的线性范围内对AFB1进行了灵敏检测。此外，将该传感器成功应用于食品中AFB1的检测，结果与ID-LC-MS/MS的检测结果更加一致。级联信号放大策略为快速、灵敏地检测AFB1提供了新的平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

文献相关原料

公司名称

产品信息

索莱宝

N,N,N’,N’-Tetramethylenediamine (TEMED)

索莱宝

30% acrylamide solution

来源期刊

Food Control 工程技术-食品科技

CiteScore

12.20

自引率

6.70%

发文量

758

审稿时长

33 days

期刊介绍： Food Control is an international journal that provides essential information for those involved in food safety and process control. Food Control covers the below areas that relate to food process control or to food safety of human foods: • Microbial food safety and antimicrobial systems • Mycotoxins • Hazard analysis, HACCP and food safety objectives • Risk assessment, including microbial and chemical hazards • Quality assurance • Good manufacturing practices • Food process systems design and control • Food Packaging technology and materials in contact with foods • Rapid methods of analysis and detection, including sensor technology • Codes of practice, legislation and international harmonization • Consumer issues • Education, training and research needs. The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.