Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258.

IF 2.7 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY Beilstein Journal of Nanotechnology Pub Date : 2025-01-30 eCollection Date: 2025-01-01 DOI:10.3762/bjnano.16.8

Prashantkumar Siddappa Chakra, Aishwarya Banakar, Shriram Narayan Puranik, Vishwas Kaveeshwar, C R Ravikumar, Devaraja Gayathri

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Abstract

The fundamental goal of our investigation is to employ a sustainable synthesis method for zinc oxide nanoparticles (ZnO NPs), utilizing lactic acid bacteria isolated from curd as the key biological agent. Bacteria function as agents for both reduction and capping processes, which aids the synthesis of ZnO NPs. Various characterization techniques including XRD, FTIR, UV-vis, TEM, SEM-EDX, and zeta potential measurements were employed to analyze the morphology, dimensions, and elemental composition of the generated nanoparticles. The experimental outcomes confirmed the presence of hexagonal wurtzite-structured ZnO NPs with an average size of 10 nm. The colloidal system demonstrated excellent stability with a zeta potential of -60 mV. Furthermore, the synthesized nanoparticles displayed significant antibacterial activity against selected human pathogens, with the biggest inhibition zone observed against Staphylococcus aureus (22 ± 0.57 mm) and the smallest inhibition zone observed against Salmonella enterica serovar typhi (3 ± 1 mm). MTT assay revealed the promising antiproliferative potential of ZnO NPs, with an average IC₅₀ value of 98.53 µg/mL. Additionally, the NPs were photocatalytically and electrochemically analyzed, indicating their potential use in cancer research as well as in coating and drug delivery applications.

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植物乳酸杆菌GP258合成氧化锌纳米颗粒的表征

本研究的基本目标是利用从凝乳中分离的乳酸菌作为关键的生物制剂，建立一种可持续合成氧化锌纳米颗粒（ZnO NPs）的方法。细菌在还原和封盖过程中都起作用，这有助于ZnO NPs的合成。采用XRD、FTIR、UV-vis、TEM、SEM-EDX和zeta电位等多种表征技术分析了纳米颗粒的形貌、尺寸和元素组成。实验结果证实了六方纤锌矿结构ZnO NPs的存在，其平均尺寸为10 nm。胶体体系具有良好的稳定性，zeta电位为-60 mV。此外，合成的纳米颗粒对选定的人类病原体具有显著的抗菌活性，对金黄色葡萄球菌的抑制范围最大（22±0.57 mm），对伤寒沙门氏菌的抑制范围最小（3±1 mm）。MTT实验显示ZnO NPs具有良好的抗增殖潜力，平均IC50值为98.53µg/mL。此外，对NPs进行了光催化和电化学分析，表明它们在癌症研究以及涂层和药物递送应用中的潜在用途。

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来源期刊

Beilstein Journal of Nanotechnology NANOSCIENCE & NANOTECHNOLOGY-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

5.70

自引率

3.20%

发文量

109

审稿时长

2 months

期刊介绍： The Beilstein Journal of Nanotechnology is an international, peer-reviewed, Open Access journal. It provides a unique platform for rapid publication without any charges (free for author and reader) – Platinum Open Access. The content is freely accessible 365 days a year to any user worldwide. Articles are available online immediately upon publication and are publicly archived in all major repositories. In addition, it provides a platform for publishing thematic issues (theme-based collections of articles) on topical issues in nanoscience and nanotechnology. The journal is published and completely funded by the Beilstein-Institut, a non-profit foundation located in Frankfurt am Main, Germany. The editor-in-chief is Professor Thomas Schimmel – Karlsruhe Institute of Technology. He is supported by more than 20 associate editors who are responsible for a particular subject area within the scope of the journal.