Marker assisted pyramiding of major resistance genes of tomato leaf curl and late blight diseases for stabilising tomato production.

Abstract

Globally, tomato leaf curl disease (ToLCD) caused by begomoviruses and late blight disease caused by Phytophthora infestans are important limiting factors for tomato production. Development of disease resistant cultivars is an important objective of tomato breeding programmes. Resistance genes such as Ty2 and Ty3 against ToLCD, and Ph2 and Ph3 resistant genes against late blight were utilized in this study to develop tomato lines with ToLCD and late blight resistance. Two F₂ populations derived from the crosses viz., VRT4-20-18 × (LA3152 × LA4286) and LA3152 × VRT-78-2 were used as base material. Marker assisted selection was employed throughout the generation advancement programme to select plants with the targeted genes. Gene based molecular markers AW910upF2R3, Ty3-SCAR and Ph3SCAR were used to select genotypes containing the Ty2, Ty3 and Ph3 genes, respectively. The dTG-63/HinfI CAPS marker linked to Ph2 gene was used to select the Ph2 gene containing genotypes. Advanced lines with all the four genes and/ or in different combinations with desirable agronomic traits were developed. Further, these selected genotypes were also tested for resistance to both the diseases under natural epiphytotic and artificially inoculated conditions. Lines with the Ty-3 alone or in combination with the Ty-2 performed well against ToLCD with DSI < 1, whereas lines with both the Ph2 and Ph3 genes performed well against late blight with DSI < 1. Developed advanced lines in the study can play a greater role in stabilizing tomato production by minimizing the losses caused by these diseases.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-025-01548-8.