Construction of a synthetic microbial community and its application in salt-reduced soy sauce fermentation

IF 4.5 1区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food microbiology Pub Date : 2025-01-31 DOI:10.1016/j.fm.2025.104738
Hua Liu , Wenjun Zhou , Jian Lu , Dianhui Wu , Fei Ge
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Abstract

High salt conditions negatively affect the fermentation efficiency of soy sauce and human health. This study aimed to construct a synthetic microbial community based on dominant functional microorganisms for salt-reduced soy sauce fermentation by investigating the succession and function of the microbial community during factory soy sauce fermentation. The findings revealed that the interplay between salinity and microorganisms influenced the dynamic changes of microbial communities. Furthermore, Aspergillus, Wickerhamomyces, Zygosaccharomyces, Staphylococcus, Weissella, and Tetragenococcus were analyzed to play key roles during soy sauce fermentation. Subsequently, the core strains were isolated and their strains and metabolic characteristics were evaluated. Finally, six strains (Aspergillus oryzae JQ09, Wickerhamomyces anomalus HJ07, Zygosaccharomyces rouxii JZ11, Staphylococcus carnosus QJ26, Weissella paramesenteroides ZJ19, and Tetragenococcus halophilus GY03) were employed to reconstruct the synthetic microbial community and conduct salt-reduced soy sauce fermentation. Biofortification increased the accumulation of metabolites in salt-reduced soy sauce. When the salt content was reduced to 14%, the sensory characteristics of soy sauce were closest to those of traditional soy sauce. Overall, this research presents a bottom-up approach to establish a simplified microbial community model with desired functions through deconstructing and reconstructing microbial structure and function. It has the potential to enhance the fermentation efficiency and realize the fermentation of salt-reduced traditional fermented food.

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来源期刊
Food microbiology
Food microbiology 工程技术-生物工程与应用微生物
CiteScore
11.30
自引率
3.80%
发文量
179
审稿时长
44 days
期刊介绍: Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.
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