Efficient degradation of alternariol in food by a novel isolate, Bacillus pacificus ANSB901

IF 4.5 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food microbiology Pub Date : 2025-03-07 DOI:10.1016/j.fm.2025.104770

Yanrong Liu , Xinyue Zhang , Mingxin Ma , Yongpeng Guo , Qiugang Ma , Lihong Zhao

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Abstract

Alternariol (AOH) is a mycotoxin that contaminates food and cause severe health issues for both humans and animals. Currently there are few researches on the degradation of AOH by microorganisms and enzymes. After screening 91 strains isolated from food samples and digestive tract chyme of chickens, it was found that Bacillus pacificus ANSB901 showed the highest AOH removal ratio, and its bacterial cell lysate could reduce AOH by 94% within 12 h. B. pacificus ANSB901 can efficiently eliminate AOH in distillers dried grains with soluble (DDGS), apple juice, and moldy maize. As potential food and feed supplements, B. pacificus ANSB901 appeared to show no sign of toxicity or virulence. Three AOH degradation products (C₁₄H₁₂O₅), (C₂₀H₂₀O₁₀), and (C₁₄H₁₂O₇) with low toxicity were observed. Based on the analysis of possible transformation pathways and whole genome sequencing of B. pacificus ANSB901, 15 protein candidates potentially involved in AOH degradation were screened for further validation. B. pacificus ANSB901 and its enzymes can serve as candidate biodegraders for AOH degradation in food and feed.

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来源期刊

Food microbiology 工程技术-生物工程与应用微生物

CiteScore

11.30

自引率

3.80%

发文量

179

审稿时长

44 days

期刊介绍： Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.