Analysis of high-molecular weight polyethylene glycol degradation by Pseudomonas sp.

IF 7.4 2区化学 Q1 POLYMER SCIENCE Polymer Degradation and Stability Pub Date : 2025-02-01 DOI:10.1016/j.polymdegradstab.2024.111144

M. Geisler , J. Khan , T. Heine , M.B. Ansorge-Schumacher , J. Thiele , A. Kaufmann

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Abstract

Polyethylene glycol (PEG) has widespread applications in pharmacy, medicine, and cosmetics, but its biological degradation is not completely understood, especially because of the complex analytics required for determining changes in molar mass and side or end groups, respectively. Here, we describe the activity of Pseudomonas sp. on PEG with molar masses of 6,000, 20,000 and 40,000 g mol⁻¹. The molar mass distribution after degradation was analyzed by size-exclusion chromatography (SEC) with different detection methods as well as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the differences between the results demonstrated. Further, the influence of the complex bacterial cultivation medium on analysis results was discussed. MALDI-TOF MS analysis revealed hydroxyethylene end-units on the PEG-terminus after bacterial degradation providing hints on the bacterial degradation pathway via end-unit scissioning followed by oxidation of the cleaved unit.

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假单胞菌降解高分子量聚乙二醇的研究。

聚乙二醇（PEG）在制药、医药和化妆品中有着广泛的应用，但其生物降解尚不完全清楚，特别是因为需要复杂的分析来分别确定摩尔质量和侧基或端基的变化。在这里，我们描述了假单胞菌对摩尔质量为6,000,20,000和40,000 g mol−1的PEG的活性。采用不同检测方法的粒径排除色谱（SEC）和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）对降解后的摩尔质量分布进行了分析，并分析了结果之间的差异。进一步讨论了复菌培养基对分析结果的影响。MALDI-TOF质谱分析揭示了细菌降解后peg末端的羟基乙烯末端单元，这为细菌降解途径提供了线索，该途径是通过末端单元分裂和氧化裂解单元进行的。

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来源期刊

Polymer Degradation and Stability 化学-高分子科学

CiteScore

10.10

自引率

10.20%

发文量

325

审稿时长

23 days

期刊介绍： Polymer Degradation and Stability deals with the degradation reactions and their control which are a major preoccupation of practitioners of the many and diverse aspects of modern polymer technology. Deteriorative reactions occur during processing, when polymers are subjected to heat, oxygen and mechanical stress, and during the useful life of the materials when oxygen and sunlight are the most important degradative agencies. In more specialised applications, degradation may be induced by high energy radiation, ozone, atmospheric pollutants, mechanical stress, biological action, hydrolysis and many other influences. The mechanisms of these reactions and stabilisation processes must be understood if the technology and application of polymers are to continue to advance. The reporting of investigations of this kind is therefore a major function of this journal. However there are also new developments in polymer technology in which degradation processes find positive applications. For example, photodegradable plastics are now available, the recycling of polymeric products will become increasingly important, degradation and combustion studies are involved in the definition of the fire hazards which are associated with polymeric materials and the microelectronics industry is vitally dependent upon polymer degradation in the manufacture of its circuitry. Polymer properties may also be improved by processes like curing and grafting, the chemistry of which can be closely related to that which causes physical deterioration in other circumstances.