Quercetin inhibits the biological activity of endometrial cancer by regulating autophagy: A network pharmacology analysis and cellular experimental validation

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Journal of Functional Foods Pub Date : 2025-02-01 DOI:10.1016/j.jff.2024.106657

Lingchen Yang , Hongwei Ma

{"title":"Quercetin inhibits the biological activity of endometrial cancer by regulating autophagy: A network pharmacology analysis and cellular experimental validation","authors":"Lingchen Yang , Hongwei Ma","doi":"10.1016/j.jff.2024.106657","DOIUrl":null,"url":null,"abstract":"<div><div>To investigate the effects of quercetin (Que) on the improvement of endometrial cancer (EC) and the relative associated mechanisms. Network pharmacology was used to analyze the correlation between Que. and EC. Ishikawa and HEC-1 A cell lines were used as research models in the present study. The cell lines were divided into the following groups: Normal, lentivirus (LV)-negative control (NC), Que., Que. + LV-activating transcription factor 5 (ATF5)-RNA interference (RNAi) and Que. + LV-ATF5-RNAi+mTOR agonist. Cell Counting Kit-8 and flow cytometry assays were used to evaluate cell proliferation and apoptosis; cell invasive and migratory abilities were assessed by Transwell and wound healing assays, respectively; the observation of the cell ultrastructure was performed by transmission electron microscopy; the relative protein expressions were measured by the western blot assay. Depending on results, Que. exhibited antitumor effects by stimulating autophagy via regulating the ATF5/JUN/PI3K/AKT/mTOR pathway in EC.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106657"},"PeriodicalIF":3.8000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Functional Foods","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1756464624006601","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

To investigate the effects of quercetin (Que) on the improvement of endometrial cancer (EC) and the relative associated mechanisms. Network pharmacology was used to analyze the correlation between Que. and EC. Ishikawa and HEC-1 A cell lines were used as research models in the present study. The cell lines were divided into the following groups: Normal, lentivirus (LV)-negative control (NC), Que., Que. + LV-activating transcription factor 5 (ATF5)-RNA interference (RNAi) and Que. + LV-ATF5-RNAi+mTOR agonist. Cell Counting Kit-8 and flow cytometry assays were used to evaluate cell proliferation and apoptosis; cell invasive and migratory abilities were assessed by Transwell and wound healing assays, respectively; the observation of the cell ultrastructure was performed by transmission electron microscopy; the relative protein expressions were measured by the western blot assay. Depending on results, Que. exhibited antitumor effects by stimulating autophagy via regulating the ATF5/JUN/PI3K/AKT/mTOR pathway in EC.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.