Journal of Functional Foods最新文献

Andrographolide protects against the intestinal barrier dysfunction and inflammatory response through modulating ETEC virulence factors in a mouse model of diarrhea 穿心莲内酯在腹泻小鼠模型中通过调节 ETEC 毒力因子防止肠屏障功能紊乱和炎症反应

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2024-11-17 DOI: 10.1016/j.jff.2024.106568

Peng Wang , Aoshuang Fan , Jinduo Zhao , Yangming liu , Lin Feng , Fangyu Xia , Yuting Tian , Liping Gan , Hanzhen Qiao , Erzhen Duan , Jin Huang , Sen lin , Jinrong Wang

Infectious diarrhea is a common disease that endanger both human and livestock animals. However, there are limited safety strategy for preventing Enterotoxigenic Escherichia coli (ETEC) induced diarrhea. A mouse diarrhea model was established by orally administering ETEC following andrographolide pretreatment. Pretreatment with andrographolide improved the body weight losses, diarrhea, intestinal barrier dysfunction and inflammation of ETEC infected mice. Andrographolide pretreatment protected against the ETEC-induced downregulation of intestinal Na⁺/H⁺ exchange protein 3 (Nhe3) and upregulation of cystic fibrosis transmembrane conductance regulator (Cftr) mRNA expression. Andrographolide pretreatment also reduced coliform colonization in the jejunum but did not affect fecal coliform levels in infected mice. By using an in vitro model, it was shown that andrographolide downregulated the expression of virulence factors involved in ETEC k88 colonization, as well as heat-labile and heat-stable enterotoxins. These results unveil a novel finding that andrographolide protect against ETEC infection by regulating its virulence factors.

感染性腹泻是一种危害人类和牲畜的常见疾病。然而，预防肠毒性大肠杆菌（ETEC）诱发腹泻的安全策略有限。通过口服 ETEC 并进行穿心莲内酯预处理，建立了小鼠腹泻模型。穿心莲内酯预处理可改善 ETEC 感染小鼠的体重减轻、腹泻、肠屏障功能障碍和炎症。穿心莲内酯预处理可防止 ETEC 诱导的肠道 Na+/H+ 交换蛋白 3（Nhe3）下调和囊性纤维化跨膜传导调节因子（Cftr）mRNA 表达上调。穿心莲内酯预处理还可减少空肠中的大肠菌群定植，但不会影响感染小鼠粪便中的大肠菌群水平。通过使用体外模型，研究表明穿心莲内酯下调了参与 ETEC k88 定殖的毒力因子以及热敏性和热稳定性肠毒素的表达。这些结果揭示了穿心莲内酯通过调节 ETEC 的毒力因子来防止其感染的新发现。

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引用次数: 0

L-theanine enhances the improvements of EGCG on glucose and lipid metabolism disorders in obese rats L -茶氨酸能增强 EGCG 对肥胖大鼠葡萄糖和脂质代谢紊乱的改善作用

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2024-11-17 DOI: 10.1016/j.jff.2024.106580

Guohuo Wu , Tingting Han , Mengdi Wang , Zhaofeng Du , Zhongwen Xie , Wei Gu

Epigallocatechin gallate (EGCG) and L-theanine (LTA) are two key bioactive components of tea that modulate nutrient metabolism. However, whether the combination of EGCG and LTA will result in synergistic benefits for glucose and lipid metabolism disorders is still unclear. Here, we treated leptin receptor-knockout (Lepr-KO) rats with EGCG and EGCG combined with LTA to examine how combined EGCG and LTA affect glucose and lipid metabolism disorders. Our data suggested that EGCG or EGCG plus LTA all significantly reduced serum triglycerides, total cholesterol, random blood glucose, and increased glucose tolerance, as well as alleviated fatty liver formation in Lepr-KO rats. Moreover, compared with EGCG alone, EGCG plus LTA significantly reduced random blood glucose and plasma triacylglycerols (TG) levels, and alleviated hepatic steatosis. Mechanistic analysis revealed that EGCG plus LTA improved glucose metabolism disorders by activating hepatic glucokinase (GCK) and glycogen synthase 2 (GYS2) expression, and inhibited fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD1) for lowering hepatic TG accumulation. This study demonstrated that LTA enhances the improvements of EGCG on glucose and lipid metabolism disorders in Lepr-KO rats, especially in glycometabolism.

表没食子儿茶素没食子酸酯（EGCG）和左旋茶氨酸（LTA）是茶叶中两种关键的生物活性成分，可调节营养代谢。然而，EGCG 和 LTA 的结合是否会对葡萄糖和脂质代谢紊乱产生协同作用仍不清楚。在此，我们用EGCG和EGCG与LTA联合治疗瘦素受体敲除（Lepr-KO）大鼠，以研究EGCG和LTA如何影响葡萄糖和脂质代谢紊乱。我们的数据表明，EGCG或EGCG加LTA均能显著降低Lepr-KO大鼠的血清甘油三酯、总胆固醇和随机血糖，提高糖耐量，并缓解脂肪肝的形成。此外，与单用 EGCG 相比，EGCG 加 LTA 能显著降低随机血糖和血浆三酰甘油（TG）水平，并缓解肝脂肪变性。机理分析表明，EGCG 加 LTA 可激活肝糖激酶（GCK）和糖原合成酶 2（GYS2）的表达，从而改善葡萄糖代谢紊乱，并抑制脂肪酸合成酶（FAS）和硬脂酰-CoA 去饱和酶 1（SCD1）以降低肝脏 TG 的积累。这项研究表明，LTA能增强EGCG对Lepr-KO大鼠葡萄糖和脂质代谢紊乱的改善作用，尤其是在糖代谢方面。

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引用次数: 0

Application and mechanism of natural products and nutrient elements in COVID-19 天然产品和营养元素在 COVID-19 中的应用和机理

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2024-11-17 DOI: 10.1016/j.jff.2024.106561

Lin Zhou, Yulin Tan, Lei Shi, Weibin Bai, Xiaoling Li

Coronavirus disease 2019 (COVID-19) is caused by the highly contagious virus Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which has caused hundreds of thousands of deaths worldwide. Finding more effective treatments has always been the focus of everyone’s attention. The study found that compared with western drugs, natural products have higher bioavailability and fewer side effects, which provides a good prospect for the development of natural product-based antiviral drugs against SARS-CoV-2. The role of nutritional interventions in disease management is receiving increasing attention. Therefore, we conducted a thorough analysis of the latest literature on the effects of natural products and nutrients on COVID-19, based on reports from both domestic and international sources, to elucidate their positive role in maintaining human health. In conclusion, this paper emphasizes the potential role of natural products and nutritional interventions in the treatment of SARS-CoV-2, and explores more efficient and safer SARS-CoV-2 response strategies.

冠状病毒病 2019（COVID-19）是由传染性极强的病毒严重急性呼吸系统综合征冠状病毒 2（SARS-CoV-2）引起的，该病毒已在全球造成数十万人死亡。寻找更有效的治疗方法一直是大家关注的焦点。研究发现，与西药相比，天然产物具有更高的生物利用度和更少的副作用，这为开发基于天然产物的抗SARS-CoV-2病毒药物提供了良好的前景。营养干预在疾病治疗中的作用越来越受到重视。因此，我们根据国内外有关天然产物和营养素对 COVID-19 影响的报道，对最新文献进行了深入分析，以阐明其在维护人类健康方面的积极作用。总之，本文强调了天然产物和营养干预在治疗 SARS-CoV-2 中的潜在作用，并探索了更有效、更安全的 SARS-CoV-2 应对策略。

引用次数: 0

BDNF-mediated regulation of GSK-3β and PSD95 phosphorylation in anti-depressive effects of Capsosiphon fulvescens glycoproteins against chronic alcohol exposure BDNF 介导的 GSK-3β 和 PSD95 磷酸化调控对 Capsosiphon fulvescens 糖蛋白抗慢性酒精暴露的抑郁作用

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2024-11-16 DOI: 10.1016/j.jff.2024.106571

Jeong Hwan Oh, Taek-Jeong Nam

Hydrophilic glycoproteins from the alga Capsosiphon fulvescens (Cf-hGP) enhance spatial memory and reduce depression-like behavior in aging rats. However, their effects on ethanol-induced depression-like behavior in adolescent rats and underlying mechanisms remain unclear. This study investigated the neuroprotective effects of Cf-hGP in mitigating depression-like behavior caused by chronic alcohol administration (2 g/kg, 4 weeks) in rats, focusing on molecular changes in the ventral hippocampus. Chronic alcohol exposure increased glucose-regulated protein 78 (an ER stress marker), while decreasing brain-derived neurotrophic factor (BDNF) and phosphorylation of glycogen synthase kinase 3-beta (GSK-3β; Ser9) and postsynaptic density protein 95 (PSD95; Ser295). Cf-hGP administration (10 mg/kg/d) reversed these effects, restoring GSK-3β and PSD95 phosphorylation. Blockade of BDNF signaling suppressed Cf-hGP’s effects, confirming its role in BDNF-mediated neuroprotection. Cf-hGP also reduced alcohol-induced depression-like behavior, suggesting its potential as a therapeutic agent for alcohol-induced depression via modulation of ER stress and BDNF signaling pathways.

来自藻类 Capsosiphon fulvescens 的亲水性糖蛋白（Cf-hGP）能增强衰老大鼠的空间记忆并减少其抑郁样行为。然而，它们对乙醇诱导的青少年大鼠抑郁样行为的影响及其内在机制仍不清楚。本研究调查了 Cf-hGP 对减轻大鼠慢性酒精摄入（2 克/公斤，4 周）引起的抑郁样行为的神经保护作用，重点研究了腹侧海马的分子变化。慢性酒精暴露增加了葡萄糖调节蛋白 78（ER 应激标记物），同时降低了脑源性神经营养因子（BDNF）、糖原合酶激酶 3-β（GSK-3β；Ser9）和突触后密度蛋白 95（PSD95；Ser295）的磷酸化。服用Cf-hGP（10 mg/kg/d）可逆转这些影响，恢复GSK-3β和PSD95的磷酸化。BDNF信号传导的阻断抑制了Cf-hGP的作用，证实了它在BDNF介导的神经保护中的作用。Cf-hGP还能减少酒精诱发的抑郁样行为，这表明它有可能通过调节ER应激和BDNF信号通路成为酒精诱发抑郁的治疗药物。

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引用次数: 0

2′-Fucosyllactose improved muscle health in middle-aged mice performing forced swimming exercise 2′-岩藻糖半乳糖能改善进行强迫游泳运动的中年小鼠的肌肉健康状况

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2024-11-15 DOI: 10.1016/j.jff.2024.106567

Jae Kwang Kim , Chul Soo Shin , Jong-Won Yoon , Seon-Min Jeon , Jeongsu Bang , Sae Kwang Ku , Kyung Hwan Jegal

Muscle mass and strength gradually decline from middle age, and appropriate physical activity and exercise habits during this period play a key role in preventing sarcopenia. Exercise is the most effective method for improving muscle health, and consuming appropriate dietary supplements along with exercise can boost its effects. Human milk is the most essential source for muscle growth during infancy, and 2′-fucosyllactose (2′-FL) is most abundant oligosaccharide in human milk. Therefore, we hypothesized that 2′-FL would exhibit muscle protective properties in 10-month-old aged male mice subjected to forced swimming exercises. Our findings revealed that 2′-FL administration significantly improved muscle mass, thickness, and strength of calf muscle compared to forced swimming exercise alone. Additionally, 2′-FL enhanced the hypertrophic changes in gastrocnemius and soleus. Furthermore, 2′-FL upregulated gene expression related to muscle protein synthesis (PI3K, AKT1) and muscle growth (A1R, TRPV4), while inhibiting gene expression related to muscle protein degradation (Atrogin-1, MuRF-1) and muscle growth inhibition (Myostatin, SIRT1). 2′-FL also ameliorated exercise-induced oxidative stress, restored antioxidant enzyme activity, and mitigated muscular cell damage. These findings suggest that 2′-FL may serve as a functional dietary supplement combined with exercise for muscle health.

人到中年，肌肉质量和力量会逐渐下降，这一时期适当的体力活动和锻炼习惯对预防肌肉疏松症起着关键作用。运动是改善肌肉健康最有效的方法，而在运动的同时摄入适当的膳食补充剂可以增强其效果。母乳是婴儿期肌肉生长最重要的来源，而 2′-岩藻糖聚糖（2′-FL）是母乳中含量最高的低聚糖。因此，我们假设 2′-FL 对 10 个月大的老年雄性小鼠的肌肉具有保护作用。我们的研究结果表明，与单独进行强迫游泳运动相比，服用 2′-FL 能明显改善小腿肌肉的质量、厚度和力量。此外，2′-FL 还能增强腓肠肌和比目鱼肌的肥大变化。此外，2′-FL 还能上调与肌肉蛋白质合成（PI3K、AKT1）和肌肉生长（A1R、TRPV4）相关的基因表达，同时抑制与肌肉蛋白质降解（Atrogin-1、MuRF-1）和肌肉生长抑制（Myostatin、SIRT1）相关的基因表达。2′-FL 还能改善运动引起的氧化应激，恢复抗氧化酶活性，减轻肌肉细胞损伤。这些研究结果表明，2′-FL 可作为一种功能性膳食补充剂，与运动相结合，促进肌肉健康。

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引用次数: 0

Hazel leaf polyphenols ameliorate hyperuricemia-induced renal fibrosis through regulating uric acid metabolism and inhibiting ferroptosis via the Nrf2/GPX4 signaling axis 榛叶多酚通过 Nrf2/GPX4 信号轴调节尿酸代谢和抑制铁卟啉沉积，从而改善高尿酸血症诱发的肾脏纤维化

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2024-11-14 DOI: 10.1016/j.jff.2024.106579

Xinhe Wang, Xiaowen Li, Jiarui Zhao, Zhi Lin, Jun Li, Xinyi Xu, Xinrui Zhou, Yuchen Wang, Guangfu Lv, He Lin, Zhe Lin

Hazel leaves, a by-product of hazelnut, have been consumed for health care in some countries and regions for hundreds of years. Based on our previous results, this study further investigated the protective effects of hazel leaf polyphenols (HP) on renal fibrosis induced by hyperuricemia in mice. Results showed that they significantly reduced serum UA, BUN and CRE contents, inhibited liver XOD activity. Histological examination and Western blot analysis revealed that they renal pathological changes and fibrosis were obviously improved after HP administration. Additionally, combined with TEM, they attenuated oxidative stress and mitochondrial damage, and inhibited ferroptosis by regulating the expression of related proteins. Molecular docking predicted that chlorogenic acid, p-coumaric acid, luteolin, myricetin and kaempferol may be the key active ingredients. In conclusion, HP ameliorated hyperuricemia-induced renal fibrosis through regulating uric acid production, reabsorption and excretion, and inhibiting ferroptosis through Nrf2/GPX4 signaling axis. It provides a valuable basis for the utilization of hazel leaf polyphenols as health agents in the future.

榛子叶是榛子的副产品，一些国家和地区食用榛子叶来保健已有数百年的历史。本研究在之前研究成果的基础上，进一步探讨了榛叶多酚（HP）对高尿酸血症诱导的小鼠肾脏纤维化的保护作用。结果表明，榛叶多酚能明显降低血清 UA、BUN 和 CRE 含量，抑制肝脏 XOD 活性。组织学检查和 Western 印迹分析表明，服用 HP 后肾脏病理变化和纤维化明显改善。此外，与 TEM 联用，它们还能减轻氧化应激和线粒体损伤，并通过调节相关蛋白的表达来抑制铁变态反应。分子对接预测绿原酸、对香豆酸、叶黄素、杨梅素和山奈酚可能是主要的活性成分。总之，HP通过调节尿酸的产生、重吸收和排泄，并通过Nrf2/GPX4信号轴抑制铁跃迁，从而改善高尿酸血症诱发的肾脏纤维化。这为今后利用榛叶多酚作为保健药物提供了宝贵的依据。

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引用次数: 0

Effects of sesamin on sleep quality and the antioxidative status in patients with low arousal threshold obstructive sleep apnea syndrome and those at risk of obstructive sleep apnea syndrome 芝麻素对低唤醒阈阻塞性睡眠呼吸暂停综合征患者和阻塞性睡眠呼吸暂停综合征高危人群睡眠质量和抗氧化状态的影响

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2024-11-14 DOI: 10.1016/j.jff.2024.106564

Lu-Chi Fu , Wen-Te Liu , Hitoshi Shirakawa , Yi-Hsiu Chen , Qian Xiao , Suh-Ching Yang

This study aims to investigate the beneficial effects of sesamin (94.0 mg/day) on sleep quality and antioxidative status in patients at risk of obstructive sleep apnea syndrome (OSAS) or those with a low arousal threshold for OSAS. It is a double-blind, self-controlled, crossover study. After 8-week sesamin supplementation, Pittsburgh Sleep Quality Index (PSQI) and Epworth Sleepiness Scale (ESS) scores significantly decreased, indicating improved sleep quality and reduced daytime sleepiness symptoms. Furthermore, the antioxidant status was significantly enhanced, including elevation of glutathione peroxidase (GPX) and glutathione reductase activities, and the glutathione content, alongside a reduction in the plasma malondialdehyde content. Results of correlation analysis showed that plasma GPX activity was negatively correlated with PSQI and ESS scores, suggesting that the oxidative stress status might be related to improvements in subjective sleep quality.

本研究旨在探讨芝麻素（94.0 毫克/天）对有阻塞性睡眠呼吸暂停综合征（OSAS）风险或 OSAS 觉醒阈值较低的患者的睡眠质量和抗氧化状态的有益影响。这是一项双盲、自控、交叉研究。在补充芝麻素 8 周后，匹兹堡睡眠质量指数（PSQI）和埃普沃斯嗜睡量表（ESS）得分显著下降，表明睡眠质量提高，白天嗜睡症状减轻。此外，抗氧化状态也得到了明显改善，包括谷胱甘肽过氧化物酶（GPX）和谷胱甘肽还原酶活性的提高、谷胱甘肽含量的增加以及血浆丙二醛含量的降低。相关分析结果显示，血浆中 GPX 活性与 PSQI 和 ESS 评分呈负相关，这表明氧化应激状态可能与主观睡眠质量的改善有关。

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引用次数: 0

Jinhua Hecha – A fermented green tea improves sleep via regulating glutamatergic synapses and gut microbiota 金华黑茶--一种通过调节谷氨酸能突触和肠道微生物群改善睡眠的发酵绿茶

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2024-11-13 DOI: 10.1016/j.jff.2024.106550

Yingying Wang , Xunjie Zheng , Ke Li , Jianli Gao , Jianbo Huang , Zhaohuan Lou

Quality sleep is crucial for preserving the internal equilibrium of the body. Sleep problems have become a predominant global health concern in recent years. Jinhua Hecha (JHHC) is a fermented green tea characterized by high amino acid and low tea polyphenols. In animal experiments, the potential beneficial effects of JHHC on sleep quality were evaluated. 50 mice were categorized into five groups: the normal control (NC) group, the FFZR positive control group, the JHHCG (2.3 g/kg) group, the JHHCZ (1.6 g/kg) group, and the JHHCD (0.7 g/kg) group. The sleep experiments revealed that mice from the JHHC group exhibited a notably extended duration of sleep following the intraperitoneal injection of pentobarbital sodium, with a shortened sleep latency after injection of barbital sodium. These results suggest that JHHC is beneficial for sleep. Mechanistically, transcriptome KEGG enrichment analysis revealed that differentially expressed genes were significantly enriched in the glutamatergic synapse signaling pathway. Immunohistochemistry and multiple immunofluorescence techniques showed decreased protein expressions of GLS, VGLUT2, GluA2, GluA4, GluK1, and mGluR1 in the colon of the JHHC group. Furthermore, an increase in beneficial bacteria such as Lactobacillus, Ligilactobacillus, and Akkermansia, as well as a decrease in Alistipes, were observed in the JHHC group. These results indicate that JHHC can improve sleep, with potential mechanisms involving gut microbiota maintenance and glutamate level reduction.

高质量的睡眠对于保持身体内部平衡至关重要。近年来，睡眠问题已成为全球关注的主要健康问题。金华黑茶是一种发酵绿茶，具有高氨基酸、低茶多酚的特点。在动物实验中，我们评估了金华黑茶对睡眠质量的潜在益处。50 只小鼠被分为五组：正常对照（NC）组、FFZR 阳性对照组、JHHCG（2.3 克/千克）组、JHHCZ（1.6 克/千克）组和 JHHCD（0.7 克/千克）组。睡眠实验显示，JHHC 组小鼠在腹腔注射戊巴比妥钠后睡眠时间明显延长，而注射巴比妥钠后睡眠潜伏期缩短。这些结果表明，JHHC有益于睡眠。从机制上看，转录组 KEGG 富集分析显示，差异表达基因显著富集于谷氨酸能突触信号通路。免疫组化和多种免疫荧光技术显示，JHHC 组结肠中 GLS、VGLUT2、GluA2、GluA4、GluK1 和 mGluR1 蛋白表达量减少。此外，在 JHHC 组中还观察到乳酸杆菌、ligilactobacillus 和 Akkermansia 等有益细菌的增加以及 Alistipes 的减少。这些结果表明，JHHC 可以改善睡眠，其潜在机制涉及肠道微生物群的维持和谷氨酸水平的降低。

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引用次数: 0

Tea intervention ameliorated metabolic dysfunction caused by circadian rhythm disorders via gut microbiota-bile acid-FXR axis 茶干预通过肠道微生物群-胆汁酸-FXR 轴改善昼夜节律紊乱引起的代谢功能障碍

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2024-11-13 DOI: 10.1016/j.jff.2024.106566

Yang Wei, Siwei Miao, Kang Wei, Lanlan Peng, Xinlin Wei

With the rapid development of society and changing lifestyles, people often encounter circadian rhythm disorder (CRD). Although tea active components have shown promise in improving CRD, a comprehensive comparison of different types of tea in improving CRD is still lacking, and the mechanism of regulating CRD-induced metabolic disorders remained unclear. This study selected three representative teas (Green tea, black tea, and dark tea) and compared the ameliorative effects of different teas on CRD-induced metabolic dysfunction. Furthermore, tea intervention improved the metabolic syndrome associated with CRD mainly through decreasing relative abundance of BSH-producing microbes, increasing conjugated bile acids (BAs) that act in an antagonistic manner on intestinal farnesoid X receptor (FXR), and promoting elimination of BAs via the feces. Our results shed light into the mechanisms behind the ameliorative effect of tea intervention on metabolic dysfunction caused by CRD, and suggest that the gut microbiota-bile acid-FXR axis may be potential therapies.

随着社会的快速发展和生活方式的改变，人们经常会遇到昼夜节律紊乱（CRD）的问题。尽管茶叶中的有效成分在改善昼夜节律紊乱方面表现出良好的前景，但目前仍缺乏对不同种类茶叶在改善昼夜节律紊乱方面作用的全面比较，而调节昼夜节律紊乱引起的代谢紊乱的机制仍不清楚。本研究选择了三种具有代表性的茶叶（绿茶、红茶和黑茶），比较了不同茶叶对 CRD 诱导的代谢功能障碍的改善作用。结果表明，茶叶对CRD相关代谢综合征的改善作用主要体现在：降低BSH产生微生物的相对丰度；增加共轭胆汁酸（BAs），以拮抗肠道法尼类脂X受体（FXR）；促进BAs通过粪便排出体外。我们的研究结果揭示了茶干预对 CRD 引起的代谢功能障碍的改善作用背后的机制，并表明肠道微生物群-胆汁酸-FXR 轴可能是一种潜在的疗法。

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引用次数: 0

Saprophytic fungus (Ganoderma sessile) restrained Treg recruitment in the tumor microenvironment by down-regulating CCL28/CCR10 pathway 萎缩性真菌（无柄灵芝）通过下调CCL28/CCR10通路抑制肿瘤微环境中Treg的招募

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2024-11-13 DOI: 10.1016/j.jff.2024.106573

Lu Jin , Jianhui Fu , Xiaochen Lin , Lushuai Jin , Yingxue Guo , Xia Liu , Shaden A.M. Khalifa , Hesham R. El-Seedi , Huiying Fu , Qiyang Shou

Ganoderma sessile, a saprophytic fungus, is a traditional Chinese medicinal material used in Chinese folk medicine and cuisine for its potential to enhance cancer patients immunity in clinical practice. However, the underlying mechanisms of its therapeutic effects remain elusive. Therefore, we explored the edible value to determine the underlying mechanisms of GS through multiple breast cancer models. GS was found to suppress tumor growth and lung metastasis, and prolong survival after surgery. It significantly increased tumor-infiltrating CD44^HiCD62L^HiCD8⁺ T_CM cells while decreasing CD4⁺CD25⁺CD127⁻ Treg cells in both lymph nodes and tumors. The mechanism behind GS’s effects primarily involved modulation of the Hif1-a/CCL28 pathway associated with Treg recruitment. Furthermore, GS could reduce CCR10 levels on the surface of Tregs. Collectively, GS inhibits tumor growth and pulmonary metastasis by restraining Treg recruitment through downregulation of the CCL28/CCR10 pathway—a finding which highlights its potential as a natural anti-tumor immune agent warranting further exploration.

灵芝（Ganoderma sessile）是一种吸附性真菌，是一种传统中药材，在中国民间医药和烹饪中被广泛使用，在临床实践中具有增强癌症患者免疫力的潜力。然而，灵芝治疗效果的内在机制仍然难以捉摸。因此，我们通过多种乳腺癌模型探索了葛根素的食用价值，以确定其潜在机制。研究发现，葛根素能抑制肿瘤生长和肺转移，延长术后生存期。它能明显增加肿瘤浸润的CD44HiCD62LHiCD8+中医药细胞，同时减少淋巴结和肿瘤中的CD4+CD25+CD127-Treg细胞。GS的作用机制主要涉及调节与Treg招募相关的Hif1-a/CCL28通路。此外，GS 还能降低 Tregs 表面的 CCR10 水平。总而言之，GS通过下调CCL28/CCR10通路抑制Treg的招募，从而抑制了肿瘤的生长和肺转移--这一发现凸显了GS作为天然抗肿瘤免疫制剂的潜力，值得进一步探索。

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引用次数: 0