ASFV p30 interacts with CCAR2 and MATR3 to promote ASFV replication

IF 2.4 2区农林科学 Q3 MICROBIOLOGY Veterinary microbiology Pub Date : 2025-02-04 DOI:10.1016/j.vetmic.2025.110416

Xuefei Chu , Shengqiang Ge , Bingrong Wu , Yuanyuan Zuo , Tiangang Xu , Jiarong Yu , Lei Yin , Hailong Qu , Jinming Li , Yihong Xiao , Zhiliang Wang

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Abstract

African swine fever (ASF) is a highly contagious and lethal disease caused by the African swine fever virus (ASFV). Currently, effective vaccines are not available for the prevention and control of ASF. ASFV is susceptible to mutations as it has a large genome and encodes numerous proteins. In addition to evading the host immune response, ASFV utilizes host proteins to regulate its replication. The ASFV p30 protein is involved in virus internalization into the host cell and is expressed throughout the viral replication cycle, influencing viral replication. This study identified the host proteins that interact with p30 using mass spectrometry analysis. Immunoprecipitation analysis confirmed that the ASFV p30 protein interacted with the host proteins CCAR2 and MATR3, co-localizing with them in the cytoplasm. CCAR2 and MATR3 promoted ASFV replication. Conversely, ASFV infection upregulated the expression of CCAR2 and MATR3 in the host. Thus, the ASFV p30 protein regulates ASFV replication by interacting with CCAR2 and MATR3.

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.