Deoxyribonucleic acid relatedness amongst staphylococcus epidermidis and staphylococcus saprophyticus strains

J. Zakrzewska-Czerwinska , M. Mordarski , M. Goodfellow , G. Pulverer
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引用次数: 3

Abstract

The degree of binding was determined between DNA preparations from 65 staphylococci representing clusters defined in a numerical phenetic survey and 3H-labelled DNA from reference strains of S. epidermidis, S. haemolyticus, S. hominis, S. saprophyticus and S. warneri. The congruence between the DNA pairing and numerical phenetic data was good with S. epidermidis and S. saprophyticus being shown to be genomic species. However, some strains identified as S. epidermidis using recommended diagnostic procedures were found to belong to other taxa, notably S. simulans and S. warneri. The moles percent guanine plus cytosine content of the DNA of the test strains was within the range 27 to 34.

DNA-Hybridisierungstests an insgesamt 65 Stämmen von koagulasenegativen Staphylokokken wurden mit 3H-markierter Referenz-DNA von Typstämmen der Spezies Staphylococcus epidermidis, S. haemolyticus, S. hominis, S. saprophyticus und S. warneri durchgeführt. Zwischen den Daten der numerischen Taxonomie und den jetzigen DNA-Hybridisierungstests konnte bei den Stämmen der Spezies S. epidermidis und S. saprophyticus eine gute Übereinstimmung konstatiert werden. Einige Stämme waren allerdings falsch klassifiziert worden, sie gehören zu anderen Spezies wie S. simulans und S. warneri. Die Guanin-und Cytosin-Werte der überprüften Staphylokokken lagen zwischen 27 und 34 mol%.

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表皮葡萄球菌和腐生葡萄球菌的脱氧核糖核酸亲缘关系
通过数值表型调查确定了65种葡萄球菌的DNA与表皮葡萄球菌、溶血葡萄球菌、人源葡萄球菌、腐生葡萄球菌和沃纳氏葡萄球菌参比菌株的3h标记DNA的结合程度。结果表明,表皮葡萄球菌和腐生葡萄球菌均为基因组种。然而,根据推荐的诊断方法,一些被鉴定为表皮葡萄球菌的菌株属于其他分类群,特别是S. simulans和S. warneri。实验菌株DNA中鸟嘌呤加胞嘧啶的摩尔百分比在27 ~ 34之间。
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