Simultaneous removal of ammonia, cadmium, and oxytetracycline via a double-layer immobilized bioreactor driven by manganese redox: Optimization and potential mechanism

Abstract

The coexistence of ammonia nitrogen (NH₄⁺-N), heavy metals and antibiotics in composite polluted wastewater has garnered significant attention. This study developed a novel double-layer biological carrier using sodium alginate, diatomite, polyvinyl alcohol, manganese-modified biochar, and pyrolusite, loaded with strains YZ8 and MA23 to form an efficient bioreactor (M1). Under conditions of a hydraulic retention time of 24 h, the carbon to nitrogen ratio and pH were 1.5 and 6.5, M1 achieved an average NH₄⁺-N removal efficiency of 99 %. Additionally, the average removal efficiencies of cadmium and oxytetracycline by M1 through biosorption, co-precipitation and Mn(Ⅲ) oxidation reached 90 % and 85 %, respectively. High-throughput results indicated that M1 had a relatively high abundance of functional bacterial genera. Comparative KEGG analysis revealed that M1 promoted the expression of functional genes involved in N cycling and Mn transformation. This study offers new perspectives on tackling the issue of composite water environmental pollution.