Spatial patterns of microplastics in freshwater bivalves (Bivalvia: Unionidae and Sphaeriidae) relative to municipal wastewater effluent discharges.

Abstract

Microplastics are discharged by municipal wastewater treatment plants (WWTPs); however, their uptake by filter-feeding freshwater bivalves is poorly understood. This study examined the abundance and characteristics of microplastics in wild bivalves from five locations along a 155 km stretch of the Grand River (Ontario, Canada) in 2021-2022, including upstream and downstream of three municipal WWTPs. At each site, fingernail clams (Sphaeriidae spp., n = 5 composites), freshwater mussels (Lasmigona costata, n = 10; gill, digestive gland, and hemolymph), and surface water (n = 3) were sampled at a single timepoint. Microplastics (particles >38 μm to 5 mm) were isolated and visualized via stereomicroscopy, and a subset chemically analyzed using Fourier transform infrared spectroscopy. Fingernail clams contained the highest total blank corrected microparticle counts (35.5 ± 29.4 g-1 [mean ± SD]), mussel tissues ranged from 4.3 ± 4.2 mL-1 in hemolymph to 6.5 ± 8.1 g-1 in digestive gland, and water contained 5.5 ± 2.8 L-1. Fibers were the dominant morphology across all samples, most particles were between 80 μm and 2 mm in length and, of those analyzed chemically, 30.0% were a plastic polymer. At sites downstream of WWTP outfalls, elevated counts were only seen in mussel gills and not in other bivalve tissues or water compared with upstream samples. Although microplastics were found across all sites in both biotic and abiotic compartments, results suggest little impact of WWTP discharges on their uptake in downstream bivalves.