Effective standardization of bovine frozen semen sperm motility analysis in a multi-laboratory setting

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Reproduction Science Pub Date : 2025-02-06 DOI:10.1016/j.anireprosci.2025.107793

Leonardo F.C. Brito , Clara González-Marín, Pablo Ross

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Abstract

Sperm motility is the only functional parameter that is part of basic semen analysis, and its results have important implications for clinical practice and the production of semen doses for artificial insemination. However, substantial variations in sperm motility analysis within and across laboratories have been reported. The objective of the present study was to evaluate the precision and accuracy of sperm motility analysis in a controlled, multi-laboratory setting. Ten batches of conventional and sexed bovine frozen semen were produced, with three replicates of the same batch coded with different identification numbers. Sperm motility evaluations were performed using computer-assisted sperm analysis (CASA) systems by nine technicians across six laboratories, during three test periods conducted over one year. The overall intra-technician CV was 14.7 % for conventional semen and 13.9 % for sexed semen. Bland-Altman plots showed that the mean differences of individual test results from the overall mean were close to zero (-0.4–1.1 %), with most differences (61.5 %) ranging between ± 5 %. In conclusion, precise and accurate sperm motility results were obtained by qualified technicians when following standardized procedures and utilizing CASA systems.

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.