Development of an enzymatic aptasensor for monitoring recombinant His-tagged proteins in microbial biotechnology

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Enzyme and Microbial Technology Pub Date : 2025-02-03 DOI:10.1016/j.enzmictec.2025.110603

Mohammad Javad Jadidi , Rahman Emamzadeh , Mahboobeh Nazari , Sayed Rasoul Zaker

引用次数: 0

Abstract

The utilization of polyhistidine tags (His-tag) for the purification and analysis of recombinant proteins is a widely adopted technique in biotechnology. Considering the high costs associated with antibody-based methods, the development of cost-effective techniques for protein identification following purification could significantly lower research expenses. This study developed a novel His-tag aptasensor, combining an anti-His tag aptamer with a G-quadruplex-based DNAzyme, which demonstrates limits of detection (LODs) of 0.29 μM and 0.73 μM for a His-tagged protein in calorimetric and point-of-care assays, respectively. These LODs are significantly lower than typical protein concentrations obtained through Ni-NTA affinity chromatography, indicating that the His-tag aptasensor provides an efficient solution for in vitro analysis and post-purification monitoring of His-tagged proteins.

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.