A self-powered biosensor with cascade amplification capability facilitates ultra-sensitive detection of microRNA biomarkers

IF 10.5 1区生物学 Q1 BIOPHYSICS Biosensors and Bioelectronics Pub Date : 2025-02-08 DOI:10.1016/j.bios.2025.117233

Jing Xu , Xinqi Luo , Hanxiao Chen , Bin Guo , Lijun Jia , Fu Wang

{"title":"A self-powered biosensor with cascade amplification capability facilitates ultra-sensitive detection of microRNA biomarkers","authors":"Jing Xu , Xinqi Luo , Hanxiao Chen , Bin Guo , Lijun Jia , Fu Wang","doi":"10.1016/j.bios.2025.117233","DOIUrl":null,"url":null,"abstract":"<div><div>Sensitive microRNA (miRNA) detection is crucial for cancer diagnosis. Self-powered biosensors that are used for miRNA detection show the advantages of no external power supply, mild reaction conditions, portability, etc., but still face the challenges of low efficiency of solid electrode adsorption enzyme and insufficient enzyme active site. Here, the MnS@MoS₂ composite electrode substrate boosts enzyme load, accelerates electron transfer, and enhances detection. Catalytic hairpin self-assembly (CHA) and Hybridization chain reaction (HCR) cascade bio-signal amplification enables current signal amplification via molecular recognition. When miRNA-199a is present, CHA and HCR trigger signal cascade amplification, achieving high sensitivity and specificity while powering the system. The sensor has a 0.5 fmol/L - 100 pmol/L linear response and a 0.14 fmol/L limit of detection (LOD). Additionally, the test signal is transmitted to a smartphone interface via Bluetooth, enabling portable, enabling portable, real-time detection. Our work shows this self-powered biosensor offers a new path for ultrasensitive miRNA detection, aids rapid disease biomarker monitoring, and broadens self-powered sensor use in medical diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"275 ","pages":"Article 117233"},"PeriodicalIF":10.5000,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566325001071","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

Abstract

Sensitive microRNA (miRNA) detection is crucial for cancer diagnosis. Self-powered biosensors that are used for miRNA detection show the advantages of no external power supply, mild reaction conditions, portability, etc., but still face the challenges of low efficiency of solid electrode adsorption enzyme and insufficient enzyme active site. Here, the MnS@MoS₂ composite electrode substrate boosts enzyme load, accelerates electron transfer, and enhances detection. Catalytic hairpin self-assembly (CHA) and Hybridization chain reaction (HCR) cascade bio-signal amplification enables current signal amplification via molecular recognition. When miRNA-199a is present, CHA and HCR trigger signal cascade amplification, achieving high sensitivity and specificity while powering the system. The sensor has a 0.5 fmol/L - 100 pmol/L linear response and a 0.14 fmol/L limit of detection (LOD). Additionally, the test signal is transmitted to a smartphone interface via Bluetooth, enabling portable, enabling portable, real-time detection. Our work shows this self-powered biosensor offers a new path for ultrasensitive miRNA detection, aids rapid disease biomarker monitoring, and broadens self-powered sensor use in medical diagnosis.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

具有级联扩增能力的自供电生物传感器有助于超灵敏检测microRNA生物标志物

敏感的microRNA （miRNA）检测对于癌症诊断至关重要。用于miRNA检测的自供电生物传感器具有无需外接电源、反应条件温和、便携等优点，但仍面临固体电极吸附酶效率低、酶活性位点不足的挑战。在这里，MnS@MoS 2复合电极底物增加酶负荷，加速电子转移，并提高检测。催化发夹自组装（CHA）和杂交链反应（HCR）级联生物信号放大实现了通过分子识别放大电流信号。当miRNA-199a存在时，CHA和HCR触发信号级联放大，在为系统供电的同时实现高灵敏度和特异性。该传感器具有0.5 fmol/L - 100 pmol/L的线性响应和0.14 fmol/L的检测限（LOD）。此外，测试信号通过蓝牙传输到智能手机接口，实现便携、实时检测。我们的工作表明，这种自供电的生物传感器为超灵敏的miRNA检测提供了新的途径，有助于快速监测疾病生物标志物，并扩大了自供电传感器在医学诊断中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.