A self-powered biosensor with cascade amplification capability facilitates ultra-sensitive detection of microRNA biomarkers

IF 10.7 1区生物学 Q1 BIOPHYSICS Biosensors and Bioelectronics Pub Date : 2025-02-08 DOI:10.1016/j.bios.2025.117233

Jing Xu , Xinqi Luo , Hanxiao Chen , Bin Guo , Lijun Jia , Fu Wang

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引用次数: 0

Abstract

Sensitive microRNA (miRNA) detection is crucial for cancer diagnosis. Self-powered biosensors that are used for miRNA detection show the advantages of no external power supply, mild reaction conditions, portability, etc., but still face the challenges of low efficiency of solid electrode adsorption enzyme and insufficient enzyme active site. Here, the MnS@MoS₂ composite electrode substrate boosts enzyme load, accelerates electron transfer, and enhances detection. Catalytic hairpin self-assembly (CHA) and Hybridization chain reaction (HCR) cascade bio-signal amplification enables current signal amplification via molecular recognition. When miRNA-199a is present, CHA and HCR trigger signal cascade amplification, achieving high sensitivity and specificity while powering the system. The sensor has a 0.5 fmol/L - 100 pmol/L linear response and a 0.14 fmol/L limit of detection (LOD). Additionally, the test signal is transmitted to a smartphone interface via Bluetooth, enabling portable, enabling portable, real-time detection. Our work shows this self-powered biosensor offers a new path for ultrasensitive miRNA detection, aids rapid disease biomarker monitoring, and broadens self-powered sensor use in medical diagnosis.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.