Lysophosphatidic acid 2 alleviates deep vein thrombosis via protective endothelial barrier function.

IF 1.7 4区医学 Q2 MEDICINE, GENERAL & INTERNAL Open Medicine Pub Date : 2025-02-06 eCollection Date: 2025-01-01 DOI:10.1515/med-2024-1137

Ruifeng Bai, Xinyang Yue, Xuan Tian, Huiru Zhao, Ying Liu, Tian Li, Jun Wu

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引用次数: 0

Abstract

Background: The specific role of lysophosphatidic acid 2 (LPA₂) in deep vein thrombosis (DVT) remains unclear.

Methods: An inferior vena cava annulus retraction model of DVT was established in wild-type (WT) and global LPA₂ knockout (Lpar2 ^-/- ) mice. We examined the incidence of DVT, wet weight of thrombus, length of thrombus, assessed endothelial permeability through Evans blue dye assay in vivo, cell viability, and endothelial cell (EC) permeability of mouse inferior vena cava ECs in vitro. Proteomics, histopathology, immunohistochemistry, and western blotting were employed to investigate the role of LPA₂ in DVT.

Results: Lpar2 deficiency increased vascular endothelial permeability and promoted the progression of DVT. Histological examination revealed aggravated inflammation in the thrombus of Lpar2 ^-/- DVT mice. In vitro, Lpar2 ^-/- resulted in increased permeability of ECs. Proteomic results indicated that DVT after Lpar2 ^-/- may be related to tight junction (TJ) protein. LPA₂ agonist, 2-[4-(1,3-dioxo-1H,3H-benzoisoquinolin-2-yl)butylsulfamoyl] benzoic acid, significantly reduced vascular endothelial permeability as well as increased expression of the vascular endothelial TJ protein zonula occludens-1.

Conclusion: These data provide a novel mechanism of endothelial barrier protection of LPA₂ in DVT.

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来源期刊

Open Medicine Medicine-General Medicine

CiteScore

3.00

自引率

0.00%

发文量

153

审稿时长

20 weeks

期刊介绍： Open Medicine is an open access journal that provides users with free, instant, and continued access to all content worldwide. The primary goal of the journal has always been a focus on maintaining the high quality of its published content. Its mission is to facilitate the exchange of ideas between medical science researchers from different countries. Papers connected to all fields of medicine and public health are welcomed. Open Medicine accepts submissions of research articles, reviews, case reports, letters to editor and book reviews.