Synthesis of superparamagnetic Fe3O4-PEG400 nanoparticles: Investigation of interaction with proteins and toxicity on cells

IF 5.7 3区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY Materials Research Bulletin Pub Date : 2025-06-01 Epub Date: 2025-02-03 DOI:10.1016/j.materresbull.2025.113345

Ali Valizadeh , Aboulfazl Mirzapoor , Zahra Hallaji , Mahtab Jahanshah Talab , Bijan Ranjbar

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Abstract

Investigation of nanoparticle (NP)-protein interactions is crucial, as it provides critical insights into the behavior of NPs within biological systems. In this study, magnetite nanoparticles (Fe₃O₄ NPs) were coated with polyethylene glycol 400 (PEG400) using a novel modified chemical co-precipitation method. The structural, morphological, and magnetic properties of Fe₃O₄-PEG400 NPs were characterized. The NPs show superparamagnetism, very small size, and high colloidal stability. After that, the effect of Fe₃O₄-PEG400 NPs was investigated on proteins (human serum albumin: HSA and lysozyme: Lyso) and cells (fibroblast) using fluorescence spectroscopy, circular dichroism (CD) spectropolarimetry, and MTT assay. Results showed minimal alteration in the secondary structure of proteins but the tertiary structure of proteins shows some flexibility. Moreover, the cytotoxicity test indicates Fe₃O₄-PEG400 NPs show better biocompatibility than Fe₃O₄ NPs and has an IC₅₀ of 65 µg/mL. In summary, the biocompatibility, stability, and superparamagnetism of Fe₃O₄-PEG400 NPs enhance their potential for biomedical applications.

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超顺磁性Fe3O4-PEG400纳米粒子的合成：与蛋白质的相互作用及其对细胞的毒性研究

纳米粒子(NP)-蛋白质相互作用的研究是至关重要的，因为它提供了对生物系统中纳米粒子行为的关键见解。本研究采用一种新型的改性化学共沉淀法，用聚乙二醇400 （PEG400）包覆磁铁矿纳米颗粒（Fe3O4 NPs）。表征了Fe3O4-PEG400 NPs的结构、形态和磁性能。NPs具有超顺磁性，尺寸非常小，胶体稳定性高。利用荧光光谱法、圆二色（CD）分光光度法和MTT法研究Fe3O4-PEG400 NPs对蛋白（人血清白蛋白：HSA和溶菌酶：Lyso）和细胞（成纤维细胞）的影响。结果表明，蛋白质的二级结构变化很小，但三级结构有一定的灵活性。此外，细胞毒性实验表明Fe3O4- peg400 NPs的生物相容性优于Fe3O4 NPs， IC50为65µg/mL。综上所述，Fe3O4-PEG400 NPs的生物相容性、稳定性和超顺磁性增强了它们在生物医学上的应用潜力。

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来源期刊

Materials Research Bulletin 工程技术-材料科学：综合

CiteScore

9.80

自引率

5.60%

发文量

372

审稿时长

42 days

期刊介绍： Materials Research Bulletin is an international journal reporting high-impact research on processing-structure-property relationships in functional materials and nanomaterials with interesting electronic, magnetic, optical, thermal, mechanical or catalytic properties. Papers purely on thermodynamics or theoretical calculations (e.g., density functional theory) do not fall within the scope of the journal unless they also demonstrate a clear link to physical properties. Topics covered include functional materials (e.g., dielectrics, pyroelectrics, piezoelectrics, ferroelectrics, relaxors, thermoelectrics, etc.); electrochemistry and solid-state ionics (e.g., photovoltaics, batteries, sensors, and fuel cells); nanomaterials, graphene, and nanocomposites; luminescence and photocatalysis; crystal-structure and defect-structure analysis; novel electronics; non-crystalline solids; flexible electronics; protein-material interactions; and polymeric ion-exchange membranes.