Synthesis of superparamagnetic Fe3O4-PEG400 nanoparticles: Investigation of interaction with proteins and toxicity on cells

IF 5.3 3区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY Materials Research Bulletin Pub Date : 2025-02-03 DOI:10.1016/j.materresbull.2025.113345

Ali Valizadeh , Aboulfazl Mirzapoor , Zahra Hallaji , Mahtab Jahanshah Talab , Bijan Ranjbar

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Abstract

Investigation of nanoparticle (NP)-protein interactions is crucial, as it provides critical insights into the behavior of NPs within biological systems. In this study, magnetite nanoparticles (Fe₃O₄ NPs) were coated with polyethylene glycol 400 (PEG400) using a novel modified chemical co-precipitation method. The structural, morphological, and magnetic properties of Fe₃O₄-PEG400 NPs were characterized. The NPs show superparamagnetism, very small size, and high colloidal stability. After that, the effect of Fe₃O₄-PEG400 NPs was investigated on proteins (human serum albumin: HSA and lysozyme: Lyso) and cells (fibroblast) using fluorescence spectroscopy, circular dichroism (CD) spectropolarimetry, and MTT assay. Results showed minimal alteration in the secondary structure of proteins but the tertiary structure of proteins shows some flexibility. Moreover, the cytotoxicity test indicates Fe₃O₄-PEG400 NPs show better biocompatibility than Fe₃O₄ NPs and has an IC₅₀ of 65 µg/mL. In summary, the biocompatibility, stability, and superparamagnetism of Fe₃O₄-PEG400 NPs enhance their potential for biomedical applications.

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来源期刊

Materials Research Bulletin 工程技术-材料科学：综合

CiteScore

9.80

自引率

5.60%

发文量

372

审稿时长

42 days

期刊介绍： Materials Research Bulletin is an international journal reporting high-impact research on processing-structure-property relationships in functional materials and nanomaterials with interesting electronic, magnetic, optical, thermal, mechanical or catalytic properties. Papers purely on thermodynamics or theoretical calculations (e.g., density functional theory) do not fall within the scope of the journal unless they also demonstrate a clear link to physical properties. Topics covered include functional materials (e.g., dielectrics, pyroelectrics, piezoelectrics, ferroelectrics, relaxors, thermoelectrics, etc.); electrochemistry and solid-state ionics (e.g., photovoltaics, batteries, sensors, and fuel cells); nanomaterials, graphene, and nanocomposites; luminescence and photocatalysis; crystal-structure and defect-structure analysis; novel electronics; non-crystalline solids; flexible electronics; protein-material interactions; and polymeric ion-exchange membranes.