Genomics analysis of Vreelandella piezotolerans V23 reveals its role in D-amino acids metabolism

Abstract

D-amino acids are generally supposed to be unique metabolites existing only in bacteria. They can not only modify the bacterial cell wall and promote plant growth, but also participate in the immune regulation of mammals, which is of great significance in nature. Vreelandella piezotolerans V23, a Gram-negative and aerobic bacterium, was isolated from coastal seawater of the Yellow Sea, China. Here, we report the genome of strain V23 and its genomic characteristics to utilize D-amino acids. The genome of strain V23 consists of a single circular chromosome with a size of 3,926,051 bp and a GC content of 58.11 %. Genomic analysis revealed that strain V23 possessed various genes responsible for D-amino acids metabolism and a pathway of synthesizing peptidoglycan from D-amino acids. The results indicated that strain V23 has the capacity to utilize D-amino acids. And strain V23 has been confirmed to be able to grow up with different D-amino acids as the sole nitrogen source. This study also enhances our understanding of the physiological and metabolic characteristics, interspecific diversity of strains of Vreelandella genus, and provides a crucial foundation for further investigation of D-amino acids metabolism.