{"title":"Structural changes in the ganglionic layer of the rat cerebellar cortex due to the use of monosodium glutamate and sodium nitrite in combination.","authors":"Bohdan Kononov, Serhii Bilash, Ihor Tretiak, Maryna Kononova, Olena Pronina, Mykhailo Koptev, Angelina Pirog-Zakaznikova, Svitlana Donchenko, Yaryna Oliinichenko, Vladyslav Oleksiienko","doi":"10.1016/j.tice.2025.102760","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The issue of using many food additives in food is becoming increasingly relevant. The effect of these substances on the nervous system, namely the cerebellum, is not unrelated. There are studies on the impact of food additives individually, but their combined effect has not been studied sufficiently. Therefore, the aim of our study was to determine structural changes in rats' ganglionic layer of the cerebellar cortex under the influence of monosodium glutamate and sodium nitrite in combination.</p><p><strong>Methods: </strong>The experiment involved 84 white Wistar laboratory rats, which were divided into a control group and five experimental groups. The obtained cerebellar samples were paraffin-embedded and histological sections (3-4) μm thick were made. These sections were stained with hematoxylin, eosin, and silver impregnated by Grimmelius.</p><p><strong>Results: </strong>After calculating the average thickness of the cerebellar cortex ganglionic layer at different administration periods of monosodium glutamate and sodium nitrite in combination, significant changes were observed after week 1, where this indicator was 1.18 times less than in the control. Also, a decrease in the average thickness was observed after the 4th and 12th and a significant decrease in the 16th week of the study, namely by 1.61 times, 1.43 times and 1.77 times, respectively. It indicates substantial structural changes in the ganglionic layer.</p><p><strong>Conclusions: </strong>The study found that the ganglionic layer is formed of a single row of Purkinje cells, and they, in turn, are the main functional link in the entire grey matter of the cerebellum, which suggests that the use of a complex of food additives causes functional disorders of the cerebellum as a whole.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102760"},"PeriodicalIF":2.5000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue & cell","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.tice.2025.102760","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/7 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: The issue of using many food additives in food is becoming increasingly relevant. The effect of these substances on the nervous system, namely the cerebellum, is not unrelated. There are studies on the impact of food additives individually, but their combined effect has not been studied sufficiently. Therefore, the aim of our study was to determine structural changes in rats' ganglionic layer of the cerebellar cortex under the influence of monosodium glutamate and sodium nitrite in combination.
Methods: The experiment involved 84 white Wistar laboratory rats, which were divided into a control group and five experimental groups. The obtained cerebellar samples were paraffin-embedded and histological sections (3-4) μm thick were made. These sections were stained with hematoxylin, eosin, and silver impregnated by Grimmelius.
Results: After calculating the average thickness of the cerebellar cortex ganglionic layer at different administration periods of monosodium glutamate and sodium nitrite in combination, significant changes were observed after week 1, where this indicator was 1.18 times less than in the control. Also, a decrease in the average thickness was observed after the 4th and 12th and a significant decrease in the 16th week of the study, namely by 1.61 times, 1.43 times and 1.77 times, respectively. It indicates substantial structural changes in the ganglionic layer.
Conclusions: The study found that the ganglionic layer is formed of a single row of Purkinje cells, and they, in turn, are the main functional link in the entire grey matter of the cerebellum, which suggests that the use of a complex of food additives causes functional disorders of the cerebellum as a whole.
期刊介绍：
Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed.
Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.
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